Detailed information of TA system TA070143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 19444..19969
• Replicon: plasmid pSE81-1705-2
• Accession: NZ_CP018653
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain 81-1705

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: BTN77_RS25425
• Protein ID: WP_001159863.1
• Coordinates: 19664..19969 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: BTN77_RS25420
• Protein ID: WP_000813641.1
• Coordinates: 19444..19662 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTN77_RS25390	15096..15482	+	387	WP_000751876.1	YgiW/YdeI family stress tolerance OB fold protein	-
BTN77_RS25395	15535..15696	+	162	WP_015059604.1	hypothetical protein	-
BTN77_RS25400	15985..16974	-	990	WP_000461382.1	RepB family plasmid replication initiator protein	-
BTN77_RS25405	17468..17763	-	296	Protein_23	cytoplasmic protein	-
BTN77_RS26275	17775..18203	+	429	Protein_24	hypothetical protein	-
BTN77_RS25415	18247..18768	-	522	WP_080199465.1	hypothetical protein	-
BTN77_RS25420	19444..19662	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
BTN77_RS25425	19664..19969	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
BTN77_RS25430	19971..20261	+	291	WP_001266176.1	hypothetical protein	-
BTN77_RS25435	20258..20779	+	522	WP_099828416.1	cytoplasmic protein	-
BTN77_RS25440	20814..21596	+	783	WP_000082169.1	site-specific integrase	-
BTN77_RS25445	21605..22318	+	714	WP_000545756.1	EAL domain-containing protein	-
BTN77_RS25450	22322..22831	+	510	WP_001541544.1	CaiF/GrlA family transcriptional regulator	-
BTN77_RS25455	22825..23310	+	486	WP_058107032.1	hypothetical protein	-
BTN77_RS26150	23587..23874	-	288	WP_071530243.1	hypothetical protein	-
BTN77_RS25460	24030..24590	+	561	WP_000900095.1	inverse autotransporter beta domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..59334	59334

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T70143 WP_001159863.1 NZ_CP018653:19664-19969 [Salmonella enterica subsp. enterica serovar Enteritidis]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T70143 NZ_CP018653:19664-19969 [Salmonella enterica subsp. enterica serovar Enteritidis]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT70143 WP_000813641.1 NZ_CP018653:19444-19662 [Salmonella enterica subsp. enterica serovar Enteritidis]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT70143 NZ_CP018653:19444-19662 [Salmonella enterica subsp. enterica serovar Enteritidis]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6