Detailed information of TA system TA070141

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 19490..20015
• Replicon: plasmid pSE70-1605
• Accession: NZ_CP018641
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain 70-1605

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: BTN73_RS24030
• Protein ID: WP_001159863.1
• Coordinates: 19710..20015 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: BTN73_RS24025
• Protein ID: WP_000813641.1
• Coordinates: 19490..19708 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTN73_RS23995	15104..15490	+	387	WP_000751876.1	YgiW/YdeI family stress tolerance OB fold protein	-
BTN73_RS24000	15543..15704	+	162	WP_015059604.1	hypothetical protein	-
BTN73_RS24005	16031..17020	-	990	WP_000461382.1	RepB family plasmid replication initiator protein	-
BTN73_RS24010	17514..17809	-	296	Protein_24	cytoplasmic protein	-
BTN73_RS24545	17821..18249	+	429	Protein_25	hypothetical protein	-
BTN73_RS24020	18293..18814	-	522	WP_077681952.1	hypothetical protein	-
BTN73_RS24025	19490..19708	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
BTN73_RS24030	19710..20015	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
BTN73_RS24035	20017..20307	+	291	WP_001266176.1	hypothetical protein	-
BTN73_RS24040	20304..20825	+	522	WP_000198608.1	hypothetical protein	-
BTN73_RS24045	20860..21642	+	783	WP_000082169.1	site-specific integrase	-
BTN73_RS24050	21651..22364	+	714	WP_000545756.1	EAL domain-containing protein	-
BTN73_RS24055	22368..22877	+	510	WP_001541544.1	CaiF/GrlA family transcriptional regulator	-
BTN73_RS24060	22871..23356	+	486	WP_000905606.1	membrane protein	-
BTN73_RS24065	23633..23920	-	288	WP_071530243.1	hypothetical protein	-
BTN73_RS24070	24076..24636	+	561	WP_000900095.1	inverse autotransporter beta domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..59371	59371

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T70141 WP_001159863.1 NZ_CP018641:19710-20015 [Salmonella enterica subsp. enterica serovar Enteritidis]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T70141 NZ_CP018641:19710-20015 [Salmonella enterica subsp. enterica serovar Enteritidis]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT70141 WP_000813641.1 NZ_CP018641:19490-19708 [Salmonella enterica subsp. enterica serovar Enteritidis]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT70141 NZ_CP018641:19490-19708 [Salmonella enterica subsp. enterica serovar Enteritidis]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6