Detailed information of TA system TA070122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2355859..2356388
• Replicon: chromosome
• Accession: NZ_CP018629
• Organism: Staphylococcus aureus strain MRSA107

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BTN44_RS12670
• Protein ID: WP_000621175.1
• Coordinates: 2355859..2356221 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: BTN44_RS12675
• Protein ID: WP_000948331.1
• Coordinates: 2356218..2356388 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTN44_RS12645	2352838..2353608	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
BTN44_RS12650	2353583..2354062	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
BTN44_RS12655	2354064..2354390	-	327	WP_001052491.1	anti-sigma factor antagonist	-
BTN44_RS12660	2354509..2355510	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
BTN44_RS12670	2355859..2356221	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BTN44_RS12675	2356218..2356388	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
BTN44_RS12680	2356473..2357621	-	1149	WP_001281145.1	alanine racemase	-
BTN44_RS12685	2357687..2358046	-	360	WP_000581200.1	holo-ACP synthase	-
BTN44_RS12690	2358050..2358541	-	492	WP_001205910.1	PH domain-containing protein	-
BTN44_RS12695	2358528..2360111	-	1584	WP_001294626.1	PH domain-containing protein	-
BTN44_RS12700	2360104..2360583	-	480	WP_001287088.1	hypothetical protein	-
BTN44_RS12705	2360791..2361351	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T70122 WP_000621175.1 NZ_CP018629:c2356221-2355859 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T70122 NZ_CP018629:c2356221-2355859 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT70122 WP_000948331.1 NZ_CP018629:c2356388-2356218 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT70122 NZ_CP018629:c2356388-2356218 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1