Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 68188..68747 | Replicon | plasmid pFORC44_2 |
| Accession | NZ_CP018626 | ||
| Organism | Escherichia coli strain FORC_044 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | O82928 |
| Locus tag | FORC44_RS30570 | Protein ID | WP_000421248.1 |
| Coordinates | 68472..68747 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | C1J8K5 |
| Locus tag | FORC44_RS30565 | Protein ID | WP_001178089.1 |
| Coordinates | 68188..68472 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| FORC44_RS30510 | 63380..63940 | + | 561 | WP_000840472.1 | fertility inhibition protein FinO | - |
| FORC44_RS30515 | 64073..64285 | + | 213 | WP_001302189.1 | ANR family transcriptional regulator | - |
| FORC44_RS30520 | 64530..64991 | + | 462 | WP_001233853.1 | thermonuclease family protein | - |
| FORC44_RS30525 | 65037..65246 | + | 210 | WP_001302200.1 | hemolysin expression modulator Hha | - |
| FORC44_RS30530 | 65284..65622 | + | 339 | WP_000766796.1 | hypothetical protein | - |
| FORC44_RS30535 | 65862..66116 | + | 255 | WP_000083831.1 | replication regulatory protein RepA | - |
| FORC44_RS30545 | 66352..66426 | + | 75 | WP_001370046.1 | RepA leader peptide Tap | - |
| FORC44_RS30550 | 66419..67276 | + | 858 | WP_000130945.1 | incFII family plasmid replication initiator RepA | - |
| FORC44_RS30565 | 68188..68472 | + | 285 | WP_001178089.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| FORC44_RS30570 | 68472..68747 | + | 276 | WP_000421248.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| FORC44_RS30575 | 68842..69048 | + | 207 | WP_001105064.1 | hypothetical protein | - |
| FORC44_RS30585 | 69148..69363 | - | 216 | Protein_76 | transposase | - |
| FORC44_RS30590 | 69407..69973 | - | 567 | Protein_77 | IS3-like element IS600 family transposase | - |
| FORC44_RS30595 | 69973..70389 | - | 417 | Protein_78 | IS91 family transposase | - |
| FORC44_RS30600 | 70389..70574 | - | 186 | WP_001302179.1 | hypothetical protein | - |
| FORC44_RS30605 | 70751..72961 | + | 2211 | WP_000592771.1 | catalase/peroxidase KatP | - |
| FORC44_RS30610 | 73005..73394 | + | 390 | WP_001172748.1 | cytochrome b562 family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | exeE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP / stcE / exeE / exeG / hlyC / hlyA / hlyA / hlyA / hlyA / hlyA / hlyA / hlyA / hlyA / hlyB / hlyB | 1..107692 | 107692 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10735.42 Da Isoelectric Point: 9.9324
>T70108 WP_000421248.1 NZ_CP018626:68472-68747 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T70108 NZ_CP018626:68472-68747 [Escherichia coli]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10474.89 Da Isoelectric Point: 5.6558
>AT70108 WP_001178089.1 NZ_CP018626:68188-68472 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
Download Length: 285 bp
>AT70108 NZ_CP018626:68188-68472 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z6FAZ0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A731IHA8 |