Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 23859..24384 Replicon plasmid pFORC44_2
Accession NZ_CP018626
Organism Escherichia coli strain FORC_044

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag FORC44_RS30270 Protein ID WP_001159868.1
Coordinates 24079..24384 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag FORC44_RS30265 Protein ID WP_000813634.1
Coordinates 23859..24077 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC44_RS30225 19142..19372 + 231 WP_010891288.1 hypothetical protein -
FORC44_RS30230 19493..20233 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
FORC44_RS30235 20518..21495 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
FORC44_RS30245 21903..22103 - 201 WP_000708307.1 hypothetical protein -
FORC44_RS30250 22100..22720 - 621 WP_001248529.1 hypothetical protein -
FORC44_RS30255 22717..23400 - 684 WP_010891291.1 DNA-binding protein -
FORC44_RS30265 23859..24077 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FORC44_RS30270 24079..24384 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
FORC44_RS30275 24385..25191 + 807 WP_000016989.1 site-specific integrase -
FORC44_RS30280 25896..26114 + 219 WP_050437141.1 RepB family plasmid replication initiator protein -
FORC44_RS30285 26702..27868 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -
FORC44_RS30290 27868..28839 + 972 WP_000817031.1 ParB/RepB/Spo0J family plasmid partition protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - exeE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP / stcE / exeE / exeG / hlyC / hlyA / hlyA / hlyA / hlyA / hlyA / hlyA / hlyA / hlyA / hlyB / hlyB 1..107692 107692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T70107 WP_001159868.1 NZ_CP018626:24079-24384 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T70107 NZ_CP018626:24079-24384 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT70107 WP_000813634.1 NZ_CP018626:23859-24077 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT70107 NZ_CP018626:23859-24077 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References