Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 22133..22680 Replicon plasmid pFORC44_1
Accession NZ_CP018625
Organism Escherichia coli strain FORC_044

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag FORC44_RS29500 Protein ID WP_000969996.1
Coordinates 22399..22680 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID K4JYR0
Locus tag FORC44_RS29495 Protein ID WP_000079928.1
Coordinates 22133..22402 (+) Length 90 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC44_RS29450 17777..18310 - 534 WP_001303319.1 transcription termination factor NusG -
FORC44_RS29460 18752..19039 - 288 WP_000074849.1 conjugal transfer protein TraA -
FORC44_RS29465 18957..19220 - 264 WP_001324596.1 ash family protein -
FORC44_RS29480 20193..21224 + 1032 WP_000907875.1 replication initiation protein -
FORC44_RS29495 22133..22402 + 270 WP_000079928.1 hypothetical protein Antitoxin
FORC44_RS29500 22399..22680 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FORC44_RS29505 22720..23568 + 849 WP_001057996.1 3'-5' exonuclease -
FORC44_RS29510 23805..24239 - 435 WP_000429836.1 Hg(II)-responsive transcriptional regulator -
FORC44_RS29515 24311..24661 + 351 WP_001294663.1 mercuric transport protein MerT -
FORC44_RS29520 24675..24950 + 276 WP_000732292.1 mercury resistance system periplasmic binding protein MerP -
FORC44_RS29525 24986..25408 + 423 WP_001340589.1 organomercurial transporter MerC -
FORC44_RS29530 25460..27154 + 1695 WP_000105636.1 mercury(II) reductase -
FORC44_RS29535 27172..27534 + 363 WP_001277456.1 mercury resistance co-regulator MerD -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B - 1..111755 111755


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-92)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T70102 WP_000969996.1 NZ_CP018625:22399-22680 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T70102 NZ_CP018625:22399-22680 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10175.50 Da        Isoelectric Point: 7.3210

>AT70102 WP_000079928.1 NZ_CP018625:22133-22402 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT70102 NZ_CP018625:22133-22402 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB E9LLH3


Antitoxin

Source ID Structure
AlphaFold DB A0A5W2J4D0

References