Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | parDE/RelE-RHH |
Location | 22133..22680 | Replicon | plasmid pFORC44_1 |
Accession | NZ_CP018625 | ||
Organism | Escherichia coli strain FORC_044 |
Toxin (Protein)
Gene name | parE | Uniprot ID | E9LLH3 |
Locus tag | FORC44_RS29500 | Protein ID | WP_000969996.1 |
Coordinates | 22399..22680 (+) | Length | 94 a.a. |
Antitoxin (Protein)
Gene name | parD | Uniprot ID | K4JYR0 |
Locus tag | FORC44_RS29495 | Protein ID | WP_000079928.1 |
Coordinates | 22133..22402 (+) | Length | 90 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
FORC44_RS29450 | 17777..18310 | - | 534 | WP_001303319.1 | transcription termination factor NusG | - |
FORC44_RS29460 | 18752..19039 | - | 288 | WP_000074849.1 | conjugal transfer protein TraA | - |
FORC44_RS29465 | 18957..19220 | - | 264 | WP_001324596.1 | ash family protein | - |
FORC44_RS29480 | 20193..21224 | + | 1032 | WP_000907875.1 | replication initiation protein | - |
FORC44_RS29495 | 22133..22402 | + | 270 | WP_000079928.1 | hypothetical protein | Antitoxin |
FORC44_RS29500 | 22399..22680 | + | 282 | WP_000969996.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
FORC44_RS29505 | 22720..23568 | + | 849 | WP_001057996.1 | 3'-5' exonuclease | - |
FORC44_RS29510 | 23805..24239 | - | 435 | WP_000429836.1 | Hg(II)-responsive transcriptional regulator | - |
FORC44_RS29515 | 24311..24661 | + | 351 | WP_001294663.1 | mercuric transport protein MerT | - |
FORC44_RS29520 | 24675..24950 | + | 276 | WP_000732292.1 | mercury resistance system periplasmic binding protein MerP | - |
FORC44_RS29525 | 24986..25408 | + | 423 | WP_001340589.1 | organomercurial transporter MerC | - |
FORC44_RS29530 | 25460..27154 | + | 1695 | WP_000105636.1 | mercury(II) reductase | - |
FORC44_RS29535 | 27172..27534 | + | 363 | WP_001277456.1 | mercury resistance co-regulator MerD | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Conjugative plasmid | aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B | - | 1..111755 | 111755 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 94 a.a. Molecular weight: 10893.57 Da Isoelectric Point: 8.4615
>T70102 WP_000969996.1 NZ_CP018625:22399-22680 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP
Download Length: 282 bp
>T70102 NZ_CP018625:22399-22680 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG
Antitoxin
Download Length: 90 a.a. Molecular weight: 10175.50 Da Isoelectric Point: 7.3210
>AT70102 WP_000079928.1 NZ_CP018625:22133-22402 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK
Download Length: 270 bp
>AT70102 NZ_CP018625:22133-22402 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | E9LLH3 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A5W2J4D0 |