Detailed information of TA system TA070101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 2192390..2193038
• Replicon: chromosome
• Accession: NZ_CP018622
• Organism: Virgibacillus dokdonensis strain 21D

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A3E0WNY0
• Locus tag: A21D_RS10025
• Protein ID: WP_073013075.1
• Coordinates: 2192679..2193038 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: A21D_RS10020
• Protein ID: WP_077706817.1
• Coordinates: 2192390..2192674 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A21D_RS10005	2187698..2189219	+	1522	Protein_1973	NAD(P)H-hydrate dehydratase	-
A21D_RS10010	2189391..2190401	+	1011	WP_101933372.1	outer membrane lipoprotein carrier protein LolA	-
A21D_RS10015	2190825..2191946	+	1122	WP_101933373.1	alanine racemase	-
A21D_RS10020	2192390..2192674	+	285	WP_077706817.1	antitoxin	Antitoxin
A21D_RS10025	2192679..2193038	+	360	WP_073013075.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A21D_RS10030	2193372..2194241	+	870	WP_077702490.1	RsbT co-antagonist protein RsbRA	-
A21D_RS10035	2194238..2194603	+	366	WP_170861785.1	STAS domain-containing protein	-
A21D_RS10040	2194607..2195008	+	402	WP_073013081.1	anti-sigma regulatory factor	-
A21D_RS10045	2195219..2196232	+	1014	WP_077702491.1	PP2C family protein-serine/threonine phosphatase	-
A21D_RS10050	2196501..2196836	+	336	WP_101933374.1	STAS domain-containing protein	-
A21D_RS10055	2196833..2197318	+	486	WP_176087389.1	anti-sigma B factor RsbW	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13413.60 Da
• Isoelectric Point: 7.0134

>T70101 WP_073013075.1 NZ_CP018622:2192679-2193038 [Virgibacillus dokdonensis]
MIVQRGEVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKQYGFDRDSVILLEQI
RTLDKQRLTDKITKLDKEKMKMINQALEISLGLKDMYDQ

Nucleotide

• Length: 360 bp

>T70101 NZ_CP018622:2192679-2193038 [Virgibacillus dokdonensis]
TTGATCGTACAAAGAGGCGAAGTGTATTTCGCTGACCTTTCCCCTGTTGTTGGATCAGAGCAGGGAGGCGTCCGCCCGGT
ATTGATCCTGCAAAATGATATCGGGAATCGTTTCAGTCCTACCGTTATTGTGGCTGCAATTACAGCACAAATTCAAAAAG
CTAAACTCCCTACACATGTGGAGATTGATGCAAAACAATATGGGTTTGACCGTGATTCCGTTATTTTGCTAGAGCAGATA
CGAACACTTGATAAACAAAGGCTAACAGATAAAATTACAAAGTTAGATAAAGAAAAGATGAAAATGATTAACCAAGCATT
AGAAATCAGTTTAGGGCTGAAGGATATGTATGATCAATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10898.32 Da
• Isoelectric Point: 4.5792

>AT70101 WP_077706817.1 NZ_CP018622:2192390-2192674 [Virgibacillus dokdonensis]
MSESLQEVLVKIPKNLLNEVDGLMKYENSDLSDFICQATRNYLQHKRDEHFQQFRETMEKGYAEMGRINLTIASEAFQAE
EEAKITLERSVIGV

Nucleotide

• Length: 285 bp

>AT70101 NZ_CP018622:2192390-2192674 [Virgibacillus dokdonensis]
TTGTCAGAAAGCTTGCAAGAAGTATTAGTGAAAATACCAAAAAACCTGTTAAATGAGGTGGATGGTCTGATGAAATATGA
GAATAGTGATTTAAGTGATTTCATATGTCAGGCAACTAGAAACTATCTTCAGCATAAAAGAGATGAGCATTTTCAACAAT
TTCGCGAAACCATGGAAAAGGGCTATGCAGAAATGGGTCGCATTAATTTGACAATTGCTTCTGAAGCTTTTCAAGCGGAA
GAAGAAGCAAAAATCACCTTGGAACGCTCTGTGATCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A3E0WNY0