Detailed information of TA system TA070100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/PRK09907-MazE
• Location: 5976174..5976772
• Replicon: chromosome
• Accession: NZ_CP018620
• Organism: Paenibacillus xylanexedens strain PAMC 22703

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1L5LUV8
• Locus tag: BS614_RS26085
• Protein ID: WP_074096080.1
• Coordinates: 5976174..5976509 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: BS614_RS26090
• Protein ID: WP_036617560.1
• Coordinates: 5976506..5976772 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BS614_RS26075	5971842..5972720	-	879	WP_074096078.1	amidohydrolase family protein	-
BS614_RS26080	5972707..5975874	-	3168	WP_074096079.1	GNAT family N-acetyltransferase	-
BS614_RS26085	5976174..5976509	-	336	WP_074096080.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BS614_RS26090	5976506..5976772	-	267	WP_036617560.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BS614_RS26095	5977201..5977806	+	606	WP_036617246.1	DedA family protein	-
BS614_RS26100	5977981..5978364	-	384	WP_074096081.1	hypothetical protein	-
BS614_RS26105	5978562..5979761	-	1200	WP_074096998.1	MFS transporter	-
BS614_RS26110	5980068..5981105	-	1038	WP_074096082.1	ROK family protein	-
BS614_RS26115	5981305..5981574	-	270	WP_074096083.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-109)

Antitoxin

• Antitoxin PemI-like (5-86)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12518.44 Da
• Isoelectric Point: 5.1471

>T70100 WP_074096080.1 NZ_CP018620:c5976509-5976174 [Paenibacillus xylanexedens]
MIIPKRGDLIWLDFDPQAGHEQAGRRPVIVLSELEFNEITGFAVVCPITSQAKDYPFEVTLPEELPFSGVVLTDQLKSLD
VRQRRIQIAGHTEPTSEFMKSVLRNVRSILA

Nucleotide

• Length: 336 bp

>T70100 NZ_CP018620:c5976509-5976174 [Paenibacillus xylanexedens]
TTGATTATTCCCAAACGAGGCGATCTGATATGGCTTGACTTTGATCCTCAAGCGGGTCACGAGCAAGCCGGGAGACGACC
GGTTATTGTTCTATCGGAACTTGAATTCAATGAAATAACGGGTTTTGCCGTTGTATGTCCCATAACGAGTCAAGCCAAAG
ATTATCCTTTTGAAGTTACACTACCAGAGGAGCTGCCATTTTCAGGTGTTGTTTTGACGGATCAGTTGAAGAGTCTTGAT
GTGCGGCAACGACGTATCCAAATTGCGGGGCATACCGAACCAACCTCTGAATTTATGAAATCAGTTCTACGCAATGTCCG
TTCTATATTGGCATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10067.40 Da
• Isoelectric Point: 4.9110

>AT70100 WP_036617560.1 NZ_CP018620:c5976772-5976506 [Paenibacillus xylanexedens]
MTTATLSKWGNSSAIRIPNQLLKRLNLEEGSEIEILVTEDNELLLRPKTKPVESNEELRNHLKTLLSKVKQDTRHEEIDF
GTEGNELL

Nucleotide

• Length: 267 bp

>AT70100 NZ_CP018620:c5976772-5976506 [Paenibacillus xylanexedens]
ATGACTACAGCCACACTTAGCAAATGGGGAAATAGCAGTGCAATTCGCATTCCTAACCAATTGCTTAAGCGTCTTAATCT
GGAAGAAGGCTCGGAAATCGAGATTTTGGTGACAGAAGATAATGAATTGTTGTTGCGTCCAAAAACCAAGCCTGTAGAGT
CCAATGAGGAACTACGTAACCATCTAAAGACTTTGCTCTCTAAAGTGAAACAGGATACAAGACATGAAGAAATAGACTTT
GGTACTGAAGGGAACGAGTTACTTTGA

Structures

Toxin

• AlphaFold DB: A0A1L5LUV8