Detailed information of TA system TA070099

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2474498..2475133
• Replicon: chromosome
• Accession: NZ_CP018620
• Organism: Paenibacillus xylanexedens strain PAMC 22703

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W4B4V0
• Locus tag: BS614_RS10785
• Protein ID: WP_024630714.1
• Coordinates: 2474783..2475133 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: W4B5R5
• Locus tag: BS614_RS10780
• Protein ID: WP_017690037.1
• Coordinates: 2474498..2474779 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BS614_RS10765	2469715..2471208	-	1494	WP_074093974.1	serine hydrolase	-
BS614_RS10770	2471457..2472656	+	1200	WP_074093975.1	outer membrane lipoprotein-sorting protein	-
BS614_RS10775	2473050..2474237	+	1188	WP_074093976.1	alanine racemase	-
BS614_RS10780	2474498..2474779	+	282	WP_017690037.1	ribbon-helix-helix protein, CopG family	Antitoxin
BS614_RS10785	2474783..2475133	+	351	WP_024630714.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BS614_RS10790	2475369..2477558	+	2190	WP_074093977.1	alpha-galactosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-46)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12778.77 Da
• Isoelectric Point: 5.1184

>T70099 WP_024630714.1 NZ_CP018620:2474783-2475133 [Paenibacillus xylanexedens]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTCIVAAITAQIQKAKLPTHVEIDAAAHGFDRDSVILLEQI
RTIDKQRLTDKITHLDEETMKLVNEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T70099 NZ_CP018620:2474783-2475133 [Paenibacillus xylanexedens]
TTGATCGTAAAACGTGGTGACGTTTTTTTTGCGGATCTTTCTCCCGTTGTCGGTTCCGAGCAAGGTGGAGTCAGGCCGGT
TCTGGTGATCCAGAATGATATCGGCAACCGGTTCAGTCCAACTTGTATTGTGGCGGCTATCACCGCCCAAATCCAGAAGG
CAAAGCTGCCAACGCATGTTGAAATTGATGCGGCGGCACACGGCTTTGACCGGGACTCGGTTATTTTGCTCGAACAAATA
CGGACGATTGATAAACAGAGGCTGACTGACAAGATTACCCATCTGGACGAGGAGACCATGAAATTGGTCAACGAAGCCTT
ACAGATCAGCCTTGGTTTAATCGATTTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10737.36 Da
• Isoelectric Point: 5.8649

>AT70099 WP_017690037.1 NZ_CP018620:2474498-2474779 [Paenibacillus xylanexedens]
VANLQNTKRIMISLPDYLLQEVDGIVALENSNRSELIRQAMKLYLTERKKRYIRETMQRGYMEMAKINLTMASEAFHAEE
DADSTLDRLVSGV

Nucleotide

• Length: 282 bp

>AT70099 NZ_CP018620:2474498-2474779 [Paenibacillus xylanexedens]
GTGGCCAACTTGCAGAACACCAAGCGGATCATGATCAGTTTACCTGATTATCTTTTGCAGGAAGTGGATGGCATCGTAGC
GCTGGAGAATTCCAACCGCAGCGAATTGATTAGGCAGGCTATGAAGCTGTATTTAACGGAGCGTAAGAAACGTTACATCC
GTGAAACGATGCAGCGAGGGTACATGGAGATGGCAAAAATTAATCTGACCATGGCATCCGAAGCCTTTCATGCGGAGGAA
GATGCGGACAGCACTCTGGACCGCTTAGTTAGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: W4B4V0

Antitoxin

• AlphaFold DB: W4B5R5