Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 327272..327908 Replicon chromosome
Accession NZ_CP018574
Organism Bacillus altitudinis strain GLB197

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag BS467_RS01720 Protein ID WP_003214169.1
Coordinates 327272..327622 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag BS467_RS01725 Protein ID WP_003214273.1
Coordinates 327627..327908 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS467_RS01680 322303..322902 - 600 WP_008346897.1 SpoIIE family protein phosphatase -
BS467_RS01685 322902..323690 - 789 WP_012009000.1 RNA polymerase sigma factor SigB -
BS467_RS01690 323656..324144 - 489 WP_007496471.1 anti-sigma B factor RsbW -
BS467_RS01695 324141..324470 - 330 WP_019744215.1 anti-sigma factor antagonist -
BS467_RS01700 324530..325537 - 1008 WP_074041673.1 PP2C family protein-serine/threonine phosphatase -
BS467_RS01705 325548..325949 - 402 WP_060699688.1 anti-sigma regulatory factor -
BS467_RS01710 325952..326320 - 369 WP_003214235.1 RsbT antagonist protein RsbS -
BS467_RS01715 326325..327155 - 831 WP_008346908.1 RsbT co-antagonist protein RsbRA -
BS467_RS01720 327272..327622 - 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BS467_RS01725 327627..327908 - 282 WP_003214273.1 hypothetical protein Antitoxin
BS467_RS01730 328202..329383 - 1182 WP_008346911.1 alanine racemase -
BS467_RS01735 329512..330528 - 1017 WP_007496489.1 outer membrane lipoprotein-sorting protein -
BS467_RS01740 330689..331054 - 366 WP_019744212.1 holo-ACP synthase -
BS467_RS01745 331149..331754 + 606 WP_060832102.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T70098 WP_003214169.1 NZ_CP018574:c327622-327272 [Bacillus altitudinis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T70098 NZ_CP018574:c327622-327272 [Bacillus altitudinis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGATACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACGATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT70098 WP_003214273.1 NZ_CP018574:c327908-327627 [Bacillus altitudinis]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT70098 NZ_CP018574:c327908-327627 [Bacillus altitudinis]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGGCTTTGATGTCTGAAATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References