70097

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 962012..962571 Replicon chromosome
Accession NZ_CP018560
Organism Brucella melitensis strain BwIM_TUR_59

Toxin (Protein)


Gene name brnT Uniprot ID A0A7U8PD14
Locus tag BK205_RS05045 Protein ID WP_002966807.1
Coordinates 962326..962571 (-) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID C0RIV1
Locus tag BK205_RS05040 Protein ID WP_004683740.1
Coordinates 962012..962326 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BK205_RS04990 957717..957920 - 204 WP_004683737.1 hypothetical protein -
BK205_RS04995 957923..958138 - 216 WP_005968947.1 hypothetical protein -
BK205_RS05000 958135..958338 - 204 WP_002964088.1 hypothetical protein -
BK205_RS05005 958388..959092 + 705 WP_005968944.1 hypothetical protein -
BK205_RS05010 959089..959319 + 231 WP_002964091.1 hypothetical protein -
BK205_RS05015 959316..959591 + 276 WP_002964092.1 hypothetical protein -
BK205_RS05020 959614..960090 + 477 WP_002966806.1 hypothetical protein -
BK205_RS05025 960436..961146 + 711 WP_004683739.1 porin family protein -
BK205_RS05030 961494..961664 - 171 WP_002964095.1 DUF1508 domain-containing protein -
BK205_RS05040 962012..962326 - 315 WP_004683740.1 BrnA antitoxin family protein Antitoxin
BK205_RS05045 962326..962571 - 246 WP_002966807.1 BrnT family toxin Toxin
BK205_RS05050 962704..963342 + 639 Protein_923 tRNA-guanine transglycosylase -
BK205_RS05055 963412..964170 + 759 Protein_924 IS5 family transposase -
BK205_RS05060 964171..964863 + 693 WP_006135935.1 hypothetical protein -
BK205_RS05065 965275..966726 + 1452 WP_006135932.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - wboA / wbdA 955945..970042 14097
- flank IS/Tn - - 963412..963780 368


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9162.82 Da        Isoelectric Point: 10.3158

>T70097 WP_002966807.1 NZ_CP018560:c962571-962326 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

>T70097 NZ_CP018560:c962571-962326 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11653.48 Da        Isoelectric Point: 10.5824

>AT70097 WP_004683740.1 NZ_CP018560:c962326-962012 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT70097 NZ_CP018560:c962326-962012 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U8PD14

Antitoxin

Source ID Structure
AlphaFold DB C0RIV1

References