Detailed information of TA system TA070095

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 961913..962472
• Replicon: chromosome
• Accession: NZ_CP018546
• Organism: Brucella melitensis strain BwIM_TUR_19

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A7U8PD14
• Locus tag: BK207_RS05035
• Protein ID: WP_002966807.1
• Coordinates: 962227..962472 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: C0RIV1
• Locus tag: BK207_RS05030
• Protein ID: WP_004683740.1
• Coordinates: 961913..962227 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BK207_RS04980	957618..957821	-	204	WP_004683737.1	hypothetical protein	-
BK207_RS04985	957824..958039	-	216	WP_005968947.1	hypothetical protein	-
BK207_RS04990	958036..958239	-	204	WP_002964088.1	hypothetical protein	-
BK207_RS04995	958289..958993	+	705	WP_005968944.1	hypothetical protein	-
BK207_RS05000	958990..959220	+	231	WP_002964091.1	hypothetical protein	-
BK207_RS05005	959217..959492	+	276	WP_002964092.1	hypothetical protein	-
BK207_RS05010	959515..959991	+	477	WP_002966806.1	hypothetical protein	-
BK207_RS05015	960337..961047	+	711	WP_004683739.1	porin family protein	-
BK207_RS05020	961395..961565	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
BK207_RS05030	961913..962227	-	315	WP_004683740.1	BrnA antitoxin family protein	Antitoxin
BK207_RS05035	962227..962472	-	246	WP_002966807.1	BrnT family toxin	Toxin
BK207_RS05040	962605..963243	+	639	Protein_922	tRNA-guanine transglycosylase	-
BK207_RS05045	963313..964071	+	759	Protein_923	IS5 family transposase	-
BK207_RS05050	964072..964764	+	693	WP_006135935.1	hypothetical protein	-
BK207_RS05055	965176..966627	+	1452	WP_006135932.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	955846..969943	14097
	-	flank	IS/Tn	-	-	963313..963681	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9162.82 Da
• Isoelectric Point: 10.3158

>T70095 WP_002966807.1 NZ_CP018546:c962472-962227 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Nucleotide

• Length: 246 bp

>T70095 NZ_CP018546:c962472-962227 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11653.48 Da
• Isoelectric Point: 10.5824

>AT70095 WP_004683740.1 NZ_CP018546:c962227-961913 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT70095 NZ_CP018546:c962227-961913 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U8PD14

Antitoxin

• AlphaFold DB: C0RIV1