70092

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 961848..962407 Replicon chromosome
Accession NZ_CP018536
Organism Brucella melitensis strain BwIM_TKM_56

Toxin (Protein)


Gene name brnT Uniprot ID A0A7U8PD14
Locus tag BK183_RS05035 Protein ID WP_002966807.1
Coordinates 962162..962407 (-) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID C0RIV1
Locus tag BK183_RS05030 Protein ID WP_004683740.1
Coordinates 961848..962162 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BK183_RS04980 957553..957756 - 204 WP_004683737.1 hypothetical protein -
BK183_RS04985 957759..957974 - 216 WP_005968947.1 hypothetical protein -
BK183_RS04990 957971..958174 - 204 WP_002964088.1 hypothetical protein -
BK183_RS04995 958224..958928 + 705 WP_005968944.1 hypothetical protein -
BK183_RS05000 958925..959155 + 231 WP_002964091.1 hypothetical protein -
BK183_RS05005 959152..959427 + 276 WP_002964092.1 hypothetical protein -
BK183_RS05010 959450..959926 + 477 WP_002966806.1 hypothetical protein -
BK183_RS05015 960272..960982 + 711 WP_004683739.1 porin family protein -
BK183_RS05020 961330..961500 - 171 WP_002964095.1 DUF1508 domain-containing protein -
BK183_RS05030 961848..962162 - 315 WP_004683740.1 BrnA antitoxin family protein Antitoxin
BK183_RS05035 962162..962407 - 246 WP_002966807.1 BrnT family toxin Toxin
BK183_RS05040 962540..963178 + 639 Protein_924 tRNA-guanine transglycosylase -
BK183_RS05045 963248..964006 + 759 Protein_925 IS5 family transposase -
BK183_RS05050 964007..964699 + 693 WP_006135935.1 hypothetical protein -
BK183_RS05055 965111..966562 + 1452 WP_006135932.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - wboA / wbdA 956541..969878 13337
- flank IS/Tn - - 963248..963616 368


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9162.82 Da        Isoelectric Point: 10.3158

>T70092 WP_002966807.1 NZ_CP018536:c962407-962162 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

>T70092 NZ_CP018536:c962407-962162 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11653.48 Da        Isoelectric Point: 10.5824

>AT70092 WP_004683740.1 NZ_CP018536:c962162-961848 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT70092 NZ_CP018536:c962162-961848 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U8PD14

Antitoxin

Source ID Structure
AlphaFold DB C0RIV1

References