70087

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 961233..961792 Replicon chromosome
Accession NZ_CP018494
Organism Brucella melitensis strain BwIM_ITA_45

Toxin (Protein)


Gene name brnT Uniprot ID A0A7U8PD14
Locus tag BK187_RS05050 Protein ID WP_002966807.1
Coordinates 961547..961792 (-) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID C0RIV1
Locus tag BK187_RS05045 Protein ID WP_004683740.1
Coordinates 961233..961547 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BK187_RS04995 956928..957131 - 204 WP_004683737.1 hypothetical protein -
BK187_RS05000 957134..957349 - 216 WP_005968947.1 hypothetical protein -
BK187_RS05005 957346..957549 - 204 WP_002964088.1 hypothetical protein -
BK187_RS05010 957599..958313 + 715 Protein_914 hypothetical protein -
BK187_RS05015 958310..958540 + 231 WP_002964091.1 hypothetical protein -
BK187_RS05020 958537..958812 + 276 WP_002964092.1 hypothetical protein -
BK187_RS05025 958835..959311 + 477 WP_002966806.1 hypothetical protein -
BK187_RS05030 959657..960367 + 711 WP_004685621.1 porin family protein -
BK187_RS05035 960715..960885 - 171 WP_002964095.1 DUF1508 domain-containing protein -
BK187_RS05045 961233..961547 - 315 WP_004683740.1 BrnA antitoxin family protein Antitoxin
BK187_RS05050 961547..961792 - 246 WP_002966807.1 BrnT family toxin Toxin
BK187_RS05055 961925..962563 + 639 Protein_922 tRNA-guanine transglycosylase -
BK187_RS05060 962633..963391 + 759 Protein_923 IS5 family transposase -
BK187_RS05065 963392..964165 + 774 WP_002967634.1 hypothetical protein -
BK187_RS05070 964496..965947 + 1452 WP_004685623.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - wboA / wbdA 955156..969263 14107
- flank IS/Tn - - 962633..963001 368


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9162.82 Da        Isoelectric Point: 10.3158

>T70087 WP_002966807.1 NZ_CP018494:c961792-961547 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

>T70087 NZ_CP018494:c961792-961547 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11653.48 Da        Isoelectric Point: 10.5824

>AT70087 WP_004683740.1 NZ_CP018494:c961547-961233 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT70087 NZ_CP018494:c961547-961233 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U8PD14

Antitoxin

Source ID Structure
AlphaFold DB C0RIV1

References