70085

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 961865..962424 Replicon chromosome
Accession NZ_CP018486
Organism Brucella melitensis strain BwIM_IRN_37

Toxin (Protein)


Gene name brnT Uniprot ID A0A7U8PD14
Locus tag BK179_RS05030 Protein ID WP_002966807.1
Coordinates 962179..962424 (-) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID C0RIV1
Locus tag BK179_RS05025 Protein ID WP_004683740.1
Coordinates 961865..962179 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BK179_RS04975 957570..957773 - 204 WP_004683737.1 hypothetical protein -
BK179_RS04980 957776..957991 - 216 WP_005968947.1 hypothetical protein -
BK179_RS04985 957988..958191 - 204 WP_002964088.1 hypothetical protein -
BK179_RS04990 958241..958945 + 705 WP_005968944.1 hypothetical protein -
BK179_RS04995 958942..959172 + 231 WP_002964091.1 hypothetical protein -
BK179_RS05000 959169..959444 + 276 WP_002964092.1 hypothetical protein -
BK179_RS05005 959467..959943 + 477 WP_002966806.1 hypothetical protein -
BK179_RS05010 960289..960999 + 711 WP_004683739.1 porin family protein -
BK179_RS05015 961347..961517 - 171 WP_002964095.1 DUF1508 domain-containing protein -
BK179_RS05025 961865..962179 - 315 WP_004683740.1 BrnA antitoxin family protein Antitoxin
BK179_RS05030 962179..962424 - 246 WP_002966807.1 BrnT family toxin Toxin
BK179_RS05035 962557..963195 + 639 Protein_923 tRNA-guanine transglycosylase -
BK179_RS05040 963265..964023 + 759 Protein_924 IS5 family transposase -
BK179_RS05045 964024..964716 + 693 WP_006135935.1 hypothetical protein -
BK179_RS05050 965128..966579 + 1452 WP_006135932.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - wboA / wbdA 955798..969895 14097
- flank IS/Tn - - 963265..963633 368


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9162.82 Da        Isoelectric Point: 10.3158

>T70085 WP_002966807.1 NZ_CP018486:c962424-962179 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

>T70085 NZ_CP018486:c962424-962179 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11653.48 Da        Isoelectric Point: 10.5824

>AT70085 WP_004683740.1 NZ_CP018486:c962179-961865 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT70085 NZ_CP018486:c962179-961865 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U8PD14

Antitoxin

Source ID Structure
AlphaFold DB C0RIV1

References