Detailed information of TA system TA070081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RHH
• Location: 12634..13187
• Replicon: plasmid pLH3.4
• Accession: NZ_CP018473
• Organism: Xanthomonas perforans strain LH3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1L5QZG5
• Locus tag: BJD13_RS01115
• Protein ID: WP_054471487.1
• Coordinates: 12634..12927 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A6N8S2B8
• Locus tag: BJD13_RS01120
• Protein ID: WP_054471486.1
• Coordinates: 12915..13187 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJD13_RS01085	8656..9441	+	786	WP_061070720.1	P-type conjugative transfer protein TrbJ	-
BJD13_RS01090	9457..9723	+	267	WP_061070719.1	entry exclusion lipoprotein TrbK	-
BJD13_RS01095	9720..11380	+	1661	Protein_12	P-type conjugative transfer protein TrbL	-
BJD13_RS01100	11383..11640	+	258	WP_061070727.1	hypothetical protein	-
BJD13_RS01105	11704..11937	+	234	WP_011997484.1	type I toxin-antitoxin system ptaRNA1 family toxin	-
BJD13_RS01110	12093..12467	-	375	WP_061070726.1	hypothetical protein	-
BJD13_RS24895	12464..12634	-	171	WP_167370944.1	hypothetical protein	-
BJD13_RS01115	12634..12927	-	294	WP_054471487.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BJD13_RS01120	12915..13187	-	273	WP_054471486.1	CopG family ribbon-helix-helix protein	Antitoxin
BJD13_RS01130	13453..14634	+	1182	Protein_19	transposase family protein	-
BJD13_RS01140	14697..15461	+	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
BJD13_RS01145	15527..16012	-	486	WP_046941321.1	carboxymuconolactone decarboxylase family protein	-
BJD13_RS01150	16236..16685	+	450	Protein_22	transposase	-
BJD13_RS24100	16677..16951	-	275	Protein_23	cytochrome P460 family protein	-
BJD13_RS01160	16979..17512	-	534	WP_003830783.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..38746	38746

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-95)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10811.34 Da
• Isoelectric Point: 5.1583

>T70081 WP_054471487.1 NZ_CP018473:c12927-12634 [Xanthomonas perforans]
VPQVIVTEGAAEGLERCRRFLAVKAPDAARRAGQAIERQFLLLETAPDIGRPFPEMPELRELVIAFGDSGYVALYRHEPA
DDAVYVLAFRHQKEAGY

Nucleotide

• Length: 294 bp

>T70081 NZ_CP018473:c12927-12634 [Xanthomonas perforans]
GTGCCACAAGTAATCGTCACCGAAGGCGCGGCGGAAGGCTTGGAACGATGCCGGCGGTTCCTGGCCGTCAAGGCACCGGA
CGCCGCCAGGCGTGCCGGCCAGGCCATCGAGCGGCAATTCCTGCTGCTGGAGACGGCCCCGGACATTGGCCGGCCGTTCC
CTGAAATGCCCGAGCTGCGCGAGCTGGTGATTGCCTTCGGGGATTCCGGCTATGTGGCCCTGTACCGCCACGAACCGGCC
GACGATGCGGTGTATGTTCTGGCCTTCCGGCACCAGAAAGAGGCGGGCTACTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10563.86 Da
• Isoelectric Point: 8.0869

>AT70081 WP_054471486.1 NZ_CP018473:c13187-12915 [Xanthomonas perforans]
MATSLKIDDTLKGRVQHLASQRRRSPHWIMLEAIQQYVEREEARESFKQEALASWAAYKETGRHLTGQEVRTWLNTWGTN
DERAVPECHK

Nucleotide

• Length: 273 bp

>AT70081 NZ_CP018473:c13187-12915 [Xanthomonas perforans]
ATGGCAACGTCTCTCAAGATCGACGACACGCTGAAAGGTCGCGTCCAGCACCTCGCCAGCCAGCGCCGCCGCTCGCCCCA
CTGGATCATGCTCGAAGCGATCCAGCAGTACGTCGAGCGCGAAGAAGCCCGCGAGAGCTTCAAGCAGGAAGCCTTGGCGT
CCTGGGCGGCCTACAAGGAAACCGGCCGCCACCTGACCGGCCAGGAAGTTCGCACCTGGTTGAACACCTGGGGCACCAAT
GACGAAAGGGCGGTGCCTGAGTGCCACAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1L5QZG5

Antitoxin

• AlphaFold DB: A0A6N8S2B8