Detailed information of TA system TA070075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 19488..20029
• Replicon: plasmid pLMG930.4
• Accession: NZ_CP018465
• Organism: Xanthomonas euvesicatoria strain LMG930

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BJD11_RS01925
• Protein ID: WP_007968778.1
• Coordinates: 19488..19814 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A7W9EDN7
• Locus tag: BJD11_RS01930
• Protein ID: WP_033478260.1
• Coordinates: 19811..20029 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJD11_RS01910	14654..14851	+	198	WP_011345595.1	hypothetical protein	-
BJD11_RS01915	14855..16429	+	1575	WP_011345596.1	type IV secretion system DNA-binding domain-containing protein	-
BJD11_RS01920	16443..19418	+	2976	WP_011345597.1	conjugative relaxase	-
BJD11_RS01925	19488..19814	-	327	WP_007968778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BJD11_RS01930	19811..20029	-	219	WP_033478260.1	antitoxin MazE family protein	Antitoxin
BJD11_RS01935	20166..20582	-	417	WP_017159868.1	hypothetical protein	-
BJD11_RS01940	20636..21049	-	414	WP_041854931.1	hypothetical protein	-
BJD11_RS01945	21060..21635	-	576	WP_041854932.1	phospholipase D family protein	-
BJD11_RS01950	21678..22265	-	588	WP_011345600.1	hypothetical protein	-
BJD11_RS01955	22311..23198	-	888	WP_041854937.1	lytic transglycosylase domain-containing protein	-
BJD11_RS01960	23209..24231	-	1023	WP_007968768.1	P-type DNA transfer ATPase VirB11	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..31328	31328

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-105)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11732.81 Da
• Isoelectric Point: 9.2550

>T70075 WP_007968778.1 NZ_CP018465:c19814-19488 [Xanthomonas euvesicatoria]
MMRGDFVTIAMQGDFGKPRPALVIQANQFDEHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLLKPSQVMVDKAMTVKRDK
IGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T70075 NZ_CP018465:c19814-19488 [Xanthomonas euvesicatoria]
ATGATGCGAGGTGACTTTGTGACCATCGCCATGCAAGGCGACTTCGGAAAACCACGGCCCGCTCTGGTCATCCAGGCCAA
CCAGTTTGACGAACACACCACCGTGACGGTGCTGCCGGTGACAAGCACGCTTGTTGCTGCGCCGCTGCTGCGCATTACCG
TTCATCCAAGTACCGACAATGGCCTGCTGAAGCCGTCTCAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGTGACAAG
ATAGGTCGGGCCTTTGGGCGCGTAGACGCTGATGCGTTGGTGGAAATTGAGCGCTGCCTGGCCGTGTTCTTGGGCATCGC
TAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8476.58 Da
• Isoelectric Point: 5.0742

>AT70075 WP_033478260.1 NZ_CP018465:c20029-19811 [Xanthomonas euvesicatoria]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMEDTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 219 bp

>AT70075 NZ_CP018465:c20029-19811 [Xanthomonas euvesicatoria]
ATGGCACACGTCAATTCACGAGTTCAAAAGCACCGTGACGCCCTGCGCATGGCAGGGCTGCGTCCGGTGCAAATTTGGGT
GCCGGACACACGGCGGCCTGACTTCGCCGAGGAATGCCGCCGTCAGTGTCGTCTTGCTGCACAAGCGGACATGGAGGACA
CCGACATGCAGCGCTTCATGGATGAGGCGCTAGCAGACATGGATGGCTGGACGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7W9EDN7