Detailed information of TA system TA070074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 4256..4791
• Replicon: plasmid pLMG930.4
• Accession: NZ_CP018465
• Organism: Xanthomonas euvesicatoria strain LMG930

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q3C0B3
• Locus tag: BJD11_RS01825
• Protein ID: WP_007974210.1
• Coordinates: 4256..4576 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q3C0B2
• Locus tag: BJD11_RS01830
• Protein ID: WP_011345582.1
• Coordinates: 4576..4791 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJD11_RS01795	452..751	-	300	WP_041854933.1	VirB3 family type IV secretion system protein	-
BJD11_RS01800	748..1125	-	378	WP_011345609.1	TrbC/VirB2 family protein	-
BJD11_RS01805	1154..1435	-	282	WP_041854934.1	hypothetical protein	-
BJD11_RS01815	1939..2268	+	330	WP_007967027.1	hypothetical protein	-
BJD11_RS01820	2769..3587	+	819	WP_011345581.1	hypothetical protein	-
BJD11_RS01825	4256..4576	-	321	WP_007974210.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BJD11_RS01830	4576..4791	-	216	WP_011345582.1	antitoxin MazE family protein	Antitoxin
BJD11_RS01835	5037..5363	-	327	WP_007974207.1	hypothetical protein	-
BJD11_RS01840	5360..5986	-	627	WP_007974205.1	ParA family protein	-
BJD11_RS01850	6462..7148	+	687	WP_007968793.1	DNA-binding protein	-
BJD11_RS01855	7307..8269	+	963	WP_011345584.1	recombinase family protein	-
BJD11_RS01860	8356..8586	+	231	WP_046935364.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..31328	31328
	-	flank	IS/Tn	-	-	8674..9900	1226

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-102)

Antitoxin

• Antitoxin MazE-like (7-70)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11571.48 Da
• Isoelectric Point: 6.2890

>T70074 WP_007974210.1 NZ_CP018465:c4576-4256 [Xanthomonas euvesicatoria]
MQRGDLVTVSLQGDYGKPRPALVVQSDLLTDLESVVLCPVTSDLRNAAFRVTVEPNPANGLRALSQVMVDKLSTLPRTKI
SEPFGRLDDERMKAIDRALLLVVGVI

Nucleotide

• Length: 321 bp

>T70074 NZ_CP018465:c4576-4256 [Xanthomonas euvesicatoria]
ATGCAGCGGGGCGACCTTGTGACGGTTTCATTGCAGGGCGACTATGGCAAACCACGGCCCGCCTTGGTCGTTCAATCCGA
CTTGCTCACCGACCTGGAAAGCGTCGTGCTGTGCCCGGTGACAAGCGACCTGCGCAATGCCGCGTTCCGCGTCACCGTGG
AACCGAACCCGGCCAACGGGCTGCGCGCGCTCTCGCAGGTCATGGTGGACAAGCTCTCAACGCTGCCTCGCACCAAGATC
AGCGAACCGTTTGGCCGCCTCGATGATGAGCGTATGAAAGCGATAGACAGGGCGCTCCTGCTGGTCGTTGGCGTTATCTG
A

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8190.20 Da
• Isoelectric Point: 7.2801

>AT70074 WP_011345582.1 NZ_CP018465:c4791-4576 [Xanthomonas euvesicatoria]
MSSIHESKVARHRERMRAAGLRPVQFWVPDTRSPEFAAQVRKQCQNLKGDPAETEVLRFTEEAATHVEGWE

Nucleotide

• Length: 216 bp

>AT70074 NZ_CP018465:c4791-4576 [Xanthomonas euvesicatoria]
ATGTCATCAATTCACGAATCAAAGGTAGCCCGGCACCGCGAGCGGATGCGCGCTGCGGGGCTACGGCCTGTCCAGTTTTG
GGTGCCCGACACACGCTCTCCCGAGTTCGCGGCGCAAGTGCGCAAGCAGTGCCAAAACCTGAAGGGCGACCCGGCAGAAA
CCGAGGTTTTGCGCTTCACCGAAGAGGCCGCAACGCACGTTGAGGGCTGGGAGTGA

Structures

Toxin

• AlphaFold DB: Q3C0B3

Antitoxin

• AlphaFold DB: Q3C0B2