Detailed information of TA system TA070073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 25983..26641
• Replicon: plasmid pLMG930.1
• Accession: NZ_CP018464
• Organism: Xanthomonas euvesicatoria strain LMG930

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8PRH3
• Locus tag: BJD11_RS01010
• Protein ID: WP_011053002.1
• Coordinates: 25983..26384 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q3C002
• Locus tag: BJD11_RS01015
• Protein ID: WP_005917852.1
• Coordinates: 26381..26641 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJD11_RS00995	22995..24221	-	1227	WP_011345704.1	replication protein	-
BJD11_RS01000	24501..25688	-	1188	WP_011053001.1	zeta toxin family protein	-
BJD11_RS01005	25695..25958	-	264	WP_005917846.1	hypothetical protein	-
BJD11_RS01010	25983..26384	-	402	WP_011053002.1	type II toxin-antitoxin system VapC family toxin	Toxin
BJD11_RS01015	26381..26641	-	261	WP_005917852.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BJD11_RS01020	26831..27397	+	567	WP_011345705.1	recombinase family protein	-
BJD11_RS01025	27669..29006	-	1338	WP_011037255.1	avirulence protein	-
BJD11_RS26260	29097..30167	-	1071	WP_011345706.1	tyrosine phosphatase	-
BJD11_RS01035	30443..30865	-	423	Protein_27	resolvase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..179490	179490
	-	inside	IScluster/Tn	-	-	17405..37046	19641

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• SpoVT-AbrB domain (8-52)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14627.05 Da
• Isoelectric Point: 7.2785

>T70073 WP_011053002.1 NZ_CP018464:c26384-25983 [Xanthomonas euvesicatoria]
MKRYMLDTNTVSHLVKSHPAVSRRVIEVPMTALCMSAITGGELMFGLAKVPDAKRLQQAVMELLRRVDVLPWDGAVMERY
GSVRADLEKQGKALGSLDMLIAAHALETDSVLVTNDAAFSRVVGLTVEDWTRS

Nucleotide

• Length: 402 bp

>T70073 NZ_CP018464:c26384-25983 [Xanthomonas euvesicatoria]
ATGAAGCGGTACATGCTTGATACCAACACCGTGAGCCATCTCGTCAAGAGCCATCCGGCTGTCTCTCGACGGGTCATTGA
AGTGCCTATGACTGCGCTATGTATGTCCGCAATTACCGGTGGGGAGCTGATGTTCGGCTTGGCTAAGGTGCCAGACGCCA
AACGCCTTCAGCAAGCCGTCATGGAACTCCTGCGGCGCGTGGATGTGCTGCCTTGGGATGGCGCGGTAATGGAGCGTTAC
GGAAGCGTAAGAGCGGACCTAGAAAAGCAAGGTAAAGCGCTTGGGTCGCTCGACATGCTTATCGCAGCACATGCACTGGA
AACGGATTCTGTCCTCGTTACCAATGACGCCGCATTTAGTCGTGTTGTTGGTTTGACCGTGGAAGATTGGACACGCAGCT
AA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9770.95 Da
• Isoelectric Point: 5.0941

>AT70073 WP_005917852.1 NZ_CP018464:c26641-26381 [Xanthomonas euvesicatoria]
VSQVAKLFINGRSQAVRLPAAYRFTEKEVFIRQDPETGDVILSRKPATWEDFFIALKSADVPANFLDAAERNQGTQDRDP
FAGWRE

Nucleotide

• Length: 261 bp

>AT70073 NZ_CP018464:c26641-26381 [Xanthomonas euvesicatoria]
GTGAGCCAAGTTGCCAAGCTGTTCATCAATGGCCGCAGCCAGGCAGTCCGCTTGCCTGCCGCCTATCGCTTTACCGAGAA
AGAGGTTTTCATTCGCCAAGACCCGGAGACCGGTGACGTCATCCTGTCTCGGAAGCCTGCAACGTGGGAGGACTTCTTTA
TCGCACTCAAGAGCGCAGATGTGCCTGCTAATTTTCTAGATGCCGCCGAGCGCAATCAGGGCACGCAGGACCGTGATCCT
TTTGCGGGATGGCGCGAATGA

Structures

Toxin

• AlphaFold DB: Q8PRH3

Antitoxin

• AlphaFold DB: Q3C002