70064

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 26321..26964 Replicon plasmid unnamed
Accession NZ_CP018455
Organism Klebsiella pneumoniae strain SWU01

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag BSL40_RS29330 Protein ID WP_001044770.1
Coordinates 26321..26737 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag BSL40_RS29335 Protein ID WP_001261282.1
Coordinates 26734..26964 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSL40_RS29295 21518..22399 - 882 WP_004199234.1 carbapenem-hydrolyzing class A beta-lactamase KPC-2 -
BSL40_RS29305 22725..23422 - 698 Protein_29 IS1 family transposase -
BSL40_RS29320 23678..24700 - 1023 WP_000361404.1 helicase UvrD -
BSL40_RS29325 24685..26247 - 1563 WP_004206609.1 AAA family ATPase -
BSL40_RS29330 26321..26737 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
BSL40_RS29335 26734..26964 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BSL40_RS29340 26921..27382 + 462 WP_014343465.1 hypothetical protein -
BSL40_RS29350 27543..28487 + 945 WP_011977810.1 hypothetical protein -
BSL40_RS29355 28524..28916 + 393 WP_011977811.1 hypothetical protein -
BSL40_RS29360 28974..29495 + 522 WP_013214008.1 hypothetical protein -
BSL40_RS29365 29541..29744 + 204 WP_011977813.1 hypothetical protein -
BSL40_RS29370 29774..30778 + 1005 WP_011977814.1 hypothetical protein -
BSL40_RS29380 30962..31741 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-65 / blaSHV-12 / blaKPC-2 / rmtB / blaTEM-1B - 1..162552 162552


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T70064 WP_001044770.1 NZ_CP018455:c26737-26321 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T70064 NZ_CP018455:c26737-26321 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT70064 WP_001261282.1 NZ_CP018455:c26964-26734 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT70064 NZ_CP018455:c26964-26734 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References