Detailed information of TA system TA070045

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 19109..19752
• Replicon: plasmid pKp_Goe_070-1
• Accession: NZ_CP018451
• Organism: Klebsiella pneumoniae strain Kp_Goe_71070

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: BB748_RS28755
• Protein ID: WP_001044770.1
• Coordinates: 19336..19752 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: BB748_RS28750
• Protein ID: WP_001261282.1
• Coordinates: 19109..19339 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB748_RS28710	14681..15547	-	867	WP_004118283.1	replication initiation protein	-
BB748_RS31455	16082..16186	+	105	WP_032409716.1	hypothetical protein	-
BB748_RS28725	16315..16572	+	258	WP_000764642.1	hypothetical protein	-
BB748_RS28730	16630..17406	-	777	WP_000015958.1	site-specific integrase	-
BB748_RS28735	17403..18146	-	744	WP_000129823.1	hypothetical protein	-
BB748_RS28740	18197..18547	-	351	WP_000493378.1	hypothetical protein	-
BB748_RS28745	18691..19152	-	462	WP_072202616.1	hypothetical protein	-
BB748_RS28750	19109..19339	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BB748_RS28755	19336..19752	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
BB748_RS28760	19826..20536	+	711	WP_004152334.1	ATP-binding protein	-
BB748_RS28770	20570..21267	+	698	Protein_28	IS1 family transposase	-
BB748_RS28775	21283..22440	-	1158	Protein_29	IS1380-like element ISEc9 family transposase	-
BB748_RS28780	22628..22840	-	213	Protein_30	DUF4158 domain-containing protein	-
BB748_RS28785	23004..23561	+	558	WP_001235713.1	recombinase family protein	-
BB748_RS28790	23744..24604	+	861	WP_000027057.1	class A broad-spectrum beta-lactamase TEM-1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1C / aadA2 / cmlA1 / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / mph(A) / tet(D) / aac(3)-IIa / floR / sul3 / aac(6')-Ib / dfrA14	-	1..90684	90684

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T70045 WP_001044770.1 NZ_CP018451:19336-19752 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T70045 NZ_CP018451:19336-19752 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT70045 WP_001261282.1 NZ_CP018451:19109-19339 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT70045 NZ_CP018451:19109-19339 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3