Detailed information of TA system TA070030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1847911..1848501
• Replicon: chromosome
• Accession: NZ_CP018450
• Organism: Klebsiella pneumoniae strain Kp_Goe_71070

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: BB748_RS09485
• Protein ID: WP_023341911.1
• Coordinates: 1848169..1848501 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: BB748_RS09480
• Protein ID: WP_000288812.1
• Coordinates: 1847911..1848168 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB748_RS09460	1844246..1845994	+	1749	WP_032418698.1	hypothetical protein	-
BB748_RS09465	1846048..1846629	+	582	WP_099184326.1	hypothetical protein	-
BB748_RS09470	1846912..1847373	+	462	WP_004213450.1	hypothetical protein	-
BB748_RS09475	1847370..1847576	+	207	WP_020805021.1	helix-turn-helix domain-containing protein	-
BB748_RS09480	1847911..1848168	+	258	WP_000288812.1	antitoxin	Antitoxin
BB748_RS09485	1848169..1848501	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BB748_RS09500	1848823..1850259	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
BB748_RS09510	1850625..1852079	-	1455	WP_004148975.1	AMP nucleosidase	-
BB748_RS09515	1852209..1852454	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T70030 WP_023341911.1 NZ_CP018450:1848169-1848501 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T70030 NZ_CP018450:1848169-1848501 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT70030 WP_000288812.1 NZ_CP018450:1847911-1848168 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT70030 NZ_CP018450:1847911-1848168 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0