Detailed information of TA system TA070027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 43087..43730
• Replicon: plasmid pKp_Goe_208-1
• Accession: NZ_CP018448
• Organism: Klebsiella pneumoniae strain Kp_Goe_33208

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: BB747_RS28935
• Protein ID: WP_001044770.1
• Coordinates: 43087..43503 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: BB747_RS28940
• Protein ID: WP_001261282.1
• Coordinates: 43500..43730 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB747_RS28900	38235..39095	-	861	WP_000027057.1	class A broad-spectrum beta-lactamase TEM-1	-
BB747_RS28905	39278..39835	-	558	WP_001235713.1	recombinase family protein	-
BB747_RS28910	39999..40211	+	213	Protein_47	DUF4158 domain-containing protein	-
BB747_RS28915	40399..41556	+	1158	Protein_48	IS1380-like element ISEc9 family transposase	-
BB747_RS28920	41572..42269	-	698	Protein_49	IS1 family transposase	-
BB747_RS28930	42303..43013	-	711	WP_004152334.1	ATP-binding protein	-
BB747_RS28935	43087..43503	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
BB747_RS28940	43500..43730	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BB747_RS28945	43687..44148	+	462	WP_072202616.1	hypothetical protein	-
BB747_RS28950	44292..44642	+	351	WP_000493378.1	hypothetical protein	-
BB747_RS28955	44693..45436	+	744	WP_000129823.1	hypothetical protein	-
BB747_RS28960	45433..46209	+	777	WP_000015958.1	site-specific integrase	-
BB747_RS28965	46267..46524	-	258	WP_000764642.1	hypothetical protein	-
BB747_RS31380	46653..46757	-	105	WP_032409716.1	hypothetical protein	-
BB747_RS28980	47292..48158	+	867	WP_004118283.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(D) / mph(A) / blaTEM-1A / blaOXA-9 / ant(3'')-Ia / cmlA1 / aadA2 / blaTEM-1C / dfrA14 / aac(6')-Ib / sul3 / floR / aac(3)-IIa	-	1..90685	90685

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T70027 WP_001044770.1 NZ_CP018448:c43503-43087 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T70027 NZ_CP018448:c43503-43087 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT70027 WP_001261282.1 NZ_CP018448:c43730-43500 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT70027 NZ_CP018448:c43730-43500 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3