Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 36552..37288 Replicon plasmid pKp_Goe_917-1
Accession NZ_CP018441
Organism Klebsiella pneumoniae strain Kp_Goe_822917

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag BB746_RS27965 Protein ID WP_003026803.1
Coordinates 36806..37288 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag BB746_RS27960 Protein ID WP_003026799.1
Coordinates 36552..36818 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB746_RS27915 32614..32976 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
BB746_RS27920 33026..33376 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
BB746_RS27925 33734..34003 + 270 WP_004152102.1 hypothetical protein -
BB746_RS27930 33991..34566 + 576 WP_004152103.1 hypothetical protein -
BB746_RS27935 34597..35091 + 495 WP_004152104.1 DNA-binding protein -
BB746_RS27940 35135..35503 + 369 WP_004152105.1 hypothetical protein -
BB746_RS27945 35537..35740 + 204 WP_004152106.1 hemolysin expression modulator Hha -
BB746_RS27950 35789..36046 + 258 WP_004152107.1 hypothetical protein -
BB746_RS27955 36122..36376 + 255 WP_004152108.1 hypothetical protein -
BB746_RS27960 36552..36818 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
BB746_RS27965 36806..37288 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
BB746_RS27970 37500..38846 + 1347 WP_020314316.1 ISNCY family transposase -
BB746_RS30530 39347..40601 - 1255 Protein_44 IS3 family transposase -
BB746_RS27985 40689..41651 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / dfrA14 / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr - 1..180027 180027


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T70009 WP_003026803.1 NZ_CP018441:36806-37288 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T70009 NZ_CP018441:36806-37288 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT70009 WP_003026799.1 NZ_CP018441:36552-36818 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT70009 NZ_CP018441:36552-36818 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References