Detailed information of TA system TA069922

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 1890662..1891103
• Replicon: chromosome
• Accession: NZ_CP018412
• Organism: Burkholderia pseudomallei strain 3000015486

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: BOC58_RS09235
• Protein ID: WP_009939402.1
• Coordinates: 1890930..1891103 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: BOC58_RS09230
• Protein ID: WP_004525420.1
• Coordinates: 1890662..1890946 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOC58_RS09195	1885751..1887013	-	1263	WP_172411232.1	c-type cytochrome	-
BOC58_RS09200	1887172..1888632	-	1461	WP_085514864.1	metallophosphoesterase	-
BOC58_RS09205	1888898..1889014	+	117	Protein_1498	IS3 family transposase	-
BOC58_RS09210	1889241..1889669	-	429	Protein_1499	IS110 family transposase	-
BOC58_RS09215	1889632..1890015	-	384	Protein_1500	DDE-type integrase/transposase/recombinase	-
BOC58_RS09230	1890662..1890946	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
BOC58_RS09235	1890930..1891103	-	174	WP_009939402.1	BrnT family toxin	Toxin
BOC58_RS09240	1891824..1892420	-	597	WP_048987429.1	plasmid pRiA4b ORF-3 family protein	-
BOC58_RS09245	1892461..1894023	-	1563	WP_085539203.1	IS66 family transposase	-
BOC58_RS09250	1894053..1894400	-	348	WP_017335489.1	IS66 family insertion sequence element accessory protein TnpB	-
BOC58_RS09255	1894397..1894804	-	408	WP_085509634.1	transposase	-
BOC58_RS09260	1894911..1895138	+	228	WP_004545539.1	hypothetical protein	-
BOC58_RS40700	1895148..1895279	+	132	WP_009923452.1	bacteriophage protein Gp48	-
BOC58_RS09275	1895415..1895636	+	222	WP_004552390.1	hypothetical protein	-
BOC58_RS40050	1895603..1895902	+	300	WP_160458000.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1889632..1901012	11380
	-	inside	IScluster/Tn	-	-	1888898..1897006	8108

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T69922 WP_009939402.1 NZ_CP018412:c1891103-1890930 [Burkholderia pseudomallei]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T69922 NZ_CP018412:c1891103-1890930 [Burkholderia pseudomallei]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT69922 WP_004525420.1 NZ_CP018412:c1890946-1890662 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT69922 NZ_CP018412:c1890946-1890662 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAACCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8