Detailed information of TA system TA069918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 59563..60004
• Replicon: chromosome
• Accession: NZ_CP018411
• Organism: Burkholderia pseudomallei strain 3000015237

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: BOC57_RS21370
• Protein ID: WP_009939402.1
• Coordinates: 59831..60004 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: BOC57_RS21365
• Protein ID: WP_004525420.1
• Coordinates: 59563..59847 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOC57_RS21330	54652..55914	-	1263	WP_164977891.1	c-type cytochrome	-
BOC57_RS21335	56073..57533	-	1461	WP_020850157.1	metallophosphoesterase	-
BOC57_RS21340	57799..57915	+	117	Protein_38	IS3 family transposase	-
BOC57_RS21345	58142..58570	-	429	Protein_39	IS110 family transposase	-
BOC57_RS21350	58533..58916	-	384	Protein_40	DDE-type integrase/transposase/recombinase	-
BOC57_RS21365	59563..59847	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
BOC57_RS21370	59831..60004	-	174	WP_009939402.1	BrnT family toxin	Toxin
BOC57_RS21375	60671..60946	+	276	WP_004537565.1	bacteriophage protein Gp49	-
BOC57_RS21380	60956..61087	+	132	WP_009923452.1	bacteriophage protein Gp48	-
BOC57_RS21390	61223..61444	+	222	WP_004552390.1	hypothetical protein	-
BOC57_RS39480	61411..61710	+	300	WP_160458000.1	hypothetical protein	-
BOC57_RS21400	61941..62459	-	519	Protein_47	hypothetical protein	-
BOC57_RS21405	62562..62815	-	254	Protein_48	transposase	-
BOC57_RS21410	62955..64298	-	1344	WP_085510090.1	phosphatidylserine/phosphatidylglycerophosphate/ cardiolipin synthase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	58533..78211	19678
	-	inside	IScluster/Tn	-	-	57799..62815	5016

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T69918 WP_009939402.1 NZ_CP018411:c60004-59831 [Burkholderia pseudomallei]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T69918 NZ_CP018411:c60004-59831 [Burkholderia pseudomallei]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT69918 WP_004525420.1 NZ_CP018411:c59847-59563 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT69918 NZ_CP018411:c59847-59563 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAACCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8