Detailed information of TA system TA069914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1666320..1668073
• Replicon: chromosome
• Accession: NZ_CP018407
• Organism: Burkholderia pseudomallei strain 2013833055

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0F6L1Z5
• Locus tag: BOC54_RS29450
• Protein ID: WP_004542147.1
• Coordinates: 1666721..1668073 (+)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: BOC54_RS29445
• Protein ID: WP_043294480.1
• Coordinates: 1666320..1666724 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOC54_RS29430	1661324..1662853	-	1530	WP_085508168.1	cellulase	-
BOC54_RS29435	1662858..1665254	-	2397	WP_085508558.1	cellulose biosynthesis cyclic di-GMP-binding regulatory protein BcsB	-
BOC54_RS39850	1665719..1665982	+	264	WP_004199789.1	hypothetical protein	-
BOC54_RS29445	1666320..1666724	+	405	WP_043294480.1	helix-turn-helix domain-containing protein	Antitoxin
BOC54_RS29450	1666721..1668073	+	1353	WP_004542147.1	type II toxin-antitoxin system HipA family toxin	Toxin
BOC54_RS29455	1668230..1668682	-	453	WP_024428481.1	hypothetical protein	-
BOC54_RS39855	1668783..1668929	-	147	WP_004199790.1	hypothetical protein	-
BOC54_RS29470	1669096..1670010	-	915	WP_004198844.1	LysR family transcriptional regulator	-
BOC54_RS29475	1670177..1671130	+	954	WP_004539421.1	agmatinase	-
BOC54_RS29485	1671407..1671748	+	342	WP_004539420.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (158-406)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-71)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 49818.85 Da
• Isoelectric Point: 9.8300

>T69914 WP_004542147.1 NZ_CP018407:1666721-1668073 [Burkholderia pseudomallei]
VSARRARATRLHLWMNGLPVGYWEHARDGERLVYFDEWIGDPQGRPLSLSLPFTPGNQPYRGRLVSDYFDNLLPDSEPIR
RRIAMRYRTGGTSAFALLATLGRDCVGALQMLPPDEAPDDIERIRGHVLADADIARLLREVTSAPQAGRHAPLDDLRLSI
AGAQEKTALLRHRGRWLLPEGSTPTTHILKLPLGLVGNRRADMRTSVENEWLCARIVAAYGLPVARCDIAQFDDQKALVV
ERFDRRPSRDARWLLRLPQEDMCQATGTSALDKYQADGGPGIETIMEVLAGSEHARDDRRAFFAAQIVFWLLAATDGHAK
NFSIAHLPGNRYRSTPLYDVLSAHPVIGRGANQLPAQRARLAMGVRGKHIHYPLHQIRRRHWIAQGQRVGFAPADVDALI
DTLTARTAGVVDAVSARLPRDFPRDVADAIFSGMLGLSARLAGDAAARAP

Nucleotide

• Length: 1353 bp

>T69914 NZ_CP018407:1666721-1668073 [Burkholderia pseudomallei]
GTGAGCGCCCGCCGCGCACGCGCGACGCGCCTGCACCTGTGGATGAACGGCCTGCCCGTCGGCTACTGGGAGCACGCGCG
CGACGGCGAGCGCCTTGTCTACTTCGACGAATGGATCGGCGATCCGCAAGGCCGGCCGCTGTCGCTGTCGCTGCCGTTCA
CGCCGGGCAACCAGCCGTATCGCGGTCGGCTCGTCAGCGATTATTTCGACAACCTGCTGCCCGACAGCGAGCCGATCCGC
CGGCGAATCGCGATGCGCTACCGCACGGGCGGCACGTCCGCGTTCGCGCTGCTCGCGACGCTCGGCCGCGATTGCGTCGG
CGCGCTGCAGATGCTGCCGCCCGACGAAGCGCCGGACGACATCGAACGCATCCGCGGCCACGTGCTCGCCGACGCGGACA
TCGCGCGCCTGCTGCGCGAAGTCACGTCCGCGCCGCAGGCCGGCCGGCACGCGCCGCTCGACGATCTGCGCCTGTCGATC
GCCGGCGCGCAGGAGAAGACCGCGCTGCTGCGCCACCGCGGCCGCTGGCTGCTGCCCGAAGGGAGCACGCCGACCACGCA
CATCCTGAAGCTGCCGCTCGGGCTCGTCGGCAACCGGCGCGCCGACATGCGCACGTCGGTCGAGAACGAATGGCTGTGCG
CGCGGATCGTCGCCGCGTACGGGTTGCCCGTCGCGCGCTGCGATATCGCGCAGTTCGACGATCAGAAAGCGCTCGTCGTC
GAGCGCTTCGACCGCCGGCCGTCGCGCGACGCGCGCTGGCTCCTGCGGCTGCCGCAGGAAGACATGTGCCAGGCAACCGG
CACGTCCGCGCTCGACAAATATCAGGCCGACGGCGGCCCCGGCATCGAGACGATCATGGAAGTGCTCGCCGGCTCCGAGC
ACGCGCGGGACGATCGCCGCGCGTTCTTCGCGGCGCAGATCGTGTTCTGGCTGCTCGCCGCGACCGACGGCCACGCGAAG
AACTTCAGCATCGCGCACCTGCCCGGCAACCGCTACCGTTCGACGCCGCTTTACGACGTGCTGTCCGCGCATCCGGTCAT
CGGCCGGGGCGCGAACCAGTTGCCCGCGCAGCGCGCGCGGCTCGCGATGGGCGTGCGCGGCAAGCACATCCACTATCCGC
TGCACCAGATCCGGCGGCGGCACTGGATCGCGCAGGGCCAGCGCGTCGGCTTCGCGCCCGCCGACGTCGACGCGCTGATC
GACACGCTGACCGCGCGCACCGCGGGCGTCGTCGACGCGGTGTCGGCGCGGCTGCCGCGCGATTTTCCACGCGACGTCGC
CGATGCGATCTTCAGCGGAATGCTCGGCCTGAGCGCAAGGCTCGCCGGCGACGCGGCCGCGCGCGCGCCATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14253.55 Da
• Isoelectric Point: 11.7233

>AT69914 WP_043294480.1 NZ_CP018407:1666320-1666724 [Burkholderia pseudomallei]
MAILIEHEIKTLGQLRPILRGFRKSAGLTQAMLASRLGVTQQTYAQFEANPASASVERLFKVLRALDIELTLTLTQVYAA
PAGKDKGEVAKTAAGARAGARRAAPPASAPAPSAAGRAPRPARKRAAPKKREDW

Nucleotide

• Length: 405 bp

>AT69914 NZ_CP018407:1666320-1666724 [Burkholderia pseudomallei]
ATGGCCATCCTCATCGAGCACGAGATCAAGACACTCGGCCAGCTGCGGCCGATTCTGCGCGGCTTCCGCAAATCGGCCGG
ATTGACGCAGGCGATGCTCGCGAGCCGCCTCGGCGTCACGCAGCAGACCTACGCGCAGTTCGAGGCGAACCCGGCGTCGG
CGAGCGTCGAGCGGCTGTTCAAGGTGCTGCGCGCGCTCGACATCGAACTCACGCTCACGCTCACGCAGGTCTACGCCGCG
CCCGCGGGCAAGGACAAGGGCGAGGTTGCGAAGACGGCCGCAGGCGCGCGCGCGGGCGCGCGACGTGCCGCGCCGCCCGC
GTCCGCGCCCGCTCCGAGCGCCGCCGGGCGCGCGCCCCGCCCCGCCCGCAAGCGCGCCGCCCCGAAAAAGCGGGAGGACT
GGTGA

Structures

Toxin

• AlphaFold DB: A0A0F6L1Z5