Detailed information of TA system TA069901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 646052..647805
• Replicon: chromosome
• Accession: NZ_CP018398
• Organism: Burkholderia pseudomallei strain 2013746777

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0F6L1Z5
• Locus tag: BOC50_RS03425
• Protein ID: WP_004542147.1
• Coordinates: 646453..647805 (+)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: BOC50_RS03420
• Protein ID: WP_038736569.1
• Coordinates: 646052..646456 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOC50_RS03405	641073..642602	-	1530	WP_085561195.1	cellulase	-
BOC50_RS03410	642607..644985	-	2379	WP_085561338.1	cellulose biosynthesis cyclic di-GMP-binding regulatory protein BcsB	-
BOC50_RS38720	645450..645713	+	264	WP_004199789.1	hypothetical protein	-
BOC50_RS03420	646052..646456	+	405	WP_038736569.1	helix-turn-helix domain-containing protein	Antitoxin
BOC50_RS03425	646453..647805	+	1353	WP_004542147.1	type II toxin-antitoxin system HipA family toxin	Toxin
BOC50_RS03430	647962..648414	-	453	WP_024428481.1	hypothetical protein	-
BOC50_RS03445	648828..649742	-	915	WP_004198844.1	LysR family transcriptional regulator	-
BOC50_RS03450	649909..650862	+	954	WP_004539421.1	agmatinase	-
BOC50_RS03460	651139..651480	+	342	WP_004539420.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (158-406)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-71)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 49818.85 Da
• Isoelectric Point: 9.8300

>T69901 WP_004542147.1 NZ_CP018398:646453-647805 [Burkholderia pseudomallei]
VSARRARATRLHLWMNGLPVGYWEHARDGERLVYFDEWIGDPQGRPLSLSLPFTPGNQPYRGRLVSDYFDNLLPDSEPIR
RRIAMRYRTGGTSAFALLATLGRDCVGALQMLPPDEAPDDIERIRGHVLADADIARLLREVTSAPQAGRHAPLDDLRLSI
AGAQEKTALLRHRGRWLLPEGSTPTTHILKLPLGLVGNRRADMRTSVENEWLCARIVAAYGLPVARCDIAQFDDQKALVV
ERFDRRPSRDARWLLRLPQEDMCQATGTSALDKYQADGGPGIETIMEVLAGSEHARDDRRAFFAAQIVFWLLAATDGHAK
NFSIAHLPGNRYRSTPLYDVLSAHPVIGRGANQLPAQRARLAMGVRGKHIHYPLHQIRRRHWIAQGQRVGFAPADVDALI
DTLTARTAGVVDAVSARLPRDFPRDVADAIFSGMLGLSARLAGDAAARAP

Nucleotide

• Length: 1353 bp

>T69901 NZ_CP018398:646453-647805 [Burkholderia pseudomallei]
GTGAGCGCCCGCCGCGCACGCGCGACGCGCCTGCACCTGTGGATGAACGGCCTGCCCGTCGGCTACTGGGAGCACGCGCG
CGACGGCGAGCGCCTTGTCTACTTCGACGAATGGATCGGCGATCCGCAAGGCCGGCCGCTGTCGCTGTCGCTGCCGTTCA
CGCCGGGCAACCAGCCGTATCGCGGTCGGCTCGTCAGCGATTATTTCGACAACCTGCTGCCCGACAGCGAGCCGATCCGC
CGGCGAATCGCGATGCGCTACCGCACGGGCGGCACGTCCGCGTTCGCGCTGCTCGCGACGCTCGGCCGCGATTGCGTCGG
CGCGCTGCAGATGCTGCCGCCCGACGAAGCGCCGGACGACATCGAACGCATCCGCGGCCACGTGCTCGCCGACGCGGACA
TCGCGCGCCTGCTGCGCGAAGTCACGTCCGCGCCGCAGGCCGGCCGGCACGCGCCGCTCGACGATCTGCGCCTGTCGATC
GCCGGCGCGCAGGAGAAGACCGCGCTGCTGCGCCATCGCGGCCGCTGGCTGCTGCCCGAAGGGAGCACGCCGACCACGCA
CATCCTGAAGCTGCCGCTCGGGCTCGTCGGCAACCGGCGCGCCGACATGCGCACGTCGGTCGAGAACGAATGGCTGTGCG
CGCGGATCGTCGCCGCGTACGGGTTGCCCGTCGCGCGCTGCGACATCGCGCAGTTCGACGATCAGAAAGCGCTCGTCGTC
GAGCGCTTCGACCGCCGGCCGTCGCGCGACGCACGCTGGCTCCTGCGGCTGCCGCAGGAAGACATGTGCCAGGCAACCGG
CACGTCCGCGCTCGACAAATATCAGGCCGACGGCGGCCCCGGCATCGAGACGATCATGGAAGTGCTCGCCGGCTCCGAAC
ACGCGCGGGACGACCGCCGCGCGTTCTTCGCGGCGCAGATCGTGTTCTGGCTGCTCGCCGCGACCGACGGCCACGCGAAG
AACTTCAGCATCGCGCACCTGCCCGGCAACCGCTACCGTTCGACGCCGCTTTACGACGTGCTGTCCGCGCATCCGGTCAT
CGGCCGGGGCGCGAACCAGTTGCCCGCGCAGCGCGCGCGGCTCGCGATGGGCGTGCGCGGCAAGCACATCCACTATCCGC
TGCACCAGATCCGGCGGCGGCACTGGATCGCGCAGGGCCAGCGCGTCGGCTTCGCGCCCGCCGACGTCGACGCGCTGATC
GACACGCTGACCGCGCGCACCGCGGGCGTCGTCGACGCGGTGTCGGCGCGGCTGCCGCGCGATTTTCCACGCGACGTCGC
CGATGCGATCTTCAGCGGAATGCTCGGCCTGAGCGCAAGGCTCGCCGGCGACGCGGCCGCGCGCGCGCCATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14281.61 Da
• Isoelectric Point: 11.7233

>AT69901 WP_038736569.1 NZ_CP018398:646052-646456 [Burkholderia pseudomallei]
MAILIEHEIKTLGQLRPILRGFRKSAGLTQAMLASRLGVTQQTYAQFEANPASASVERLFKVLRALDIELTLTLTQVYAA
PAGKDKGEVAKTAAGARAGARRAVPPASAPAPSAAGRAPRPARKRAAPKKREDW

Nucleotide

• Length: 405 bp

>AT69901 NZ_CP018398:646052-646456 [Burkholderia pseudomallei]
ATGGCCATCCTCATCGAGCACGAGATCAAGACACTCGGCCAGCTGCGGCCGATTCTGCGCGGCTTCCGCAAATCGGCCGG
ATTGACGCAGGCGATGCTCGCGAGCCGCCTCGGCGTCACGCAGCAGACCTACGCGCAGTTCGAGGCGAACCCGGCGTCGG
CGAGCGTCGAGCGGCTGTTCAAGGTGCTGCGCGCGCTCGACATCGAACTCACGCTCACGCTCACGCAGGTCTACGCCGCG
CCCGCGGGCAAGGACAAGGGCGAGGTTGCGAAGACGGCCGCAGGCGCGCGCGCGGGCGCGCGACGTGCCGTGCCGCCCGC
GTCCGCGCCCGCTCCGAGCGCCGCCGGGCGCGCGCCCCGCCCCGCCCGCAAGCGCGCCGCCCCGAAAAAGCGGGAGGACT
GGTGA

Structures

Toxin

• AlphaFold DB: A0A0F6L1Z5