Detailed information of TA system TA069893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 995184..995625
• Replicon: chromosome
• Accession: NZ_CP018388
• Organism: Burkholderia pseudomallei strain 2010007509

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: BOC45_RS26230
• Protein ID: WP_009939402.1
• Coordinates: 995184..995357 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: BOC45_RS26235
• Protein ID: WP_004525420.1
• Coordinates: 995341..995625 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOC45_RS26185	991264..991611	-	348	WP_017335489.1	IS66 family insertion sequence element accessory protein TnpB	-
BOC45_RS26190	991608..992015	-	408	WP_009982080.1	transposase	-
BOC45_RS26195	992049..992615	-	567	Protein_811	prohibitin family protein	-
BOC45_RS26200	992666..993247	+	582	WP_004542548.1	hypothetical protein	-
BOC45_RS38365	993478..993777	-	300	WP_160458000.1	hypothetical protein	-
BOC45_RS26210	993744..993965	-	222	WP_004552390.1	hypothetical protein	-
BOC45_RS26220	994101..994232	-	132	WP_009923452.1	bacteriophage protein Gp48	-
BOC45_RS26225	994242..994517	-	276	WP_004537565.1	bacteriophage protein Gp49	-
BOC45_RS26230	995184..995357	+	174	WP_009939402.1	BrnT family toxin	Toxin
BOC45_RS26235	995341..995625	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
BOC45_RS26240	996029..997123	+	1095	WP_085504122.1	IS5 family transposase	-
BOC45_RS26245	997281..997628	+	348	Protein_820	DDE-type integrase/transposase/recombinase	-
BOC45_RS26250	997591..998019	+	429	Protein_821	IS110 family transposase	-
BOC45_RS26255	998246..998362	-	117	Protein_822	IS3 family transposase	-
BOC45_RS26260	998628..1000088	+	1461	WP_085514864.1	metallophosphoesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	985884..1003731	17847
	-	inside	Prophage	-	-	978441..1000088	21647

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T69893 WP_009939402.1 NZ_CP018388:995184-995357 [Burkholderia pseudomallei]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T69893 NZ_CP018388:995184-995357 [Burkholderia pseudomallei]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT69893 WP_004525420.1 NZ_CP018388:995341-995625 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT69893 NZ_CP018388:995341-995625 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAACCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8