Detailed information of TA system TA069891

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 2962386..2962827
• Replicon: chromosome
• Accession: NZ_CP018386
• Organism: Burkholderia pseudomallei strain 2008724860

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: BOC44_RS37590
• Protein ID: WP_009939402.1
• Coordinates: 2962386..2962559 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: BOC44_RS37595
• Protein ID: WP_004525420.1
• Coordinates: 2962543..2962827 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOC44_RS37555	2958805..2959233	-	429	Protein_2394	DDE-type integrase/transposase/recombinase	-
BOC44_RS40500	2959231..2959818	-	588	Protein_2395	prohibitin family protein	-
BOC44_RS37560	2959869..2960449	+	581	Protein_2396	hypothetical protein	-
BOC44_RS40505	2960680..2960979	-	300	WP_160458000.1	hypothetical protein	-
BOC44_RS37570	2960946..2961167	-	222	WP_004552390.1	hypothetical protein	-
BOC44_RS37580	2961303..2961434	-	132	WP_009923452.1	bacteriophage protein Gp48	-
BOC44_RS37585	2961444..2961719	-	276	WP_004537565.1	bacteriophage protein Gp49	-
BOC44_RS37590	2962386..2962559	+	174	WP_009939402.1	BrnT family toxin	Toxin
BOC44_RS37595	2962543..2962827	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
BOC44_RS37600	2962954..2964174	-	1221	WP_004538232.1	ISL3-like element ISBma1 family transposase	-
BOC44_RS37620	2964984..2966078	-	1095	WP_080495655.1	IS5 family transposase	-
BOC44_RS37625	2966177..2966524	+	348	Protein_2405	DDE-type integrase/transposase/recombinase	-
BOC44_RS37630	2966487..2966915	+	429	Protein_2406	IS110 family transposase	-
BOC44_RS37635	2967142..2967258	-	117	Protein_2407	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2956494..2966524	10030

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T69891 WP_009939402.1 NZ_CP018386:2962386-2962559 [Burkholderia pseudomallei]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T69891 NZ_CP018386:2962386-2962559 [Burkholderia pseudomallei]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT69891 WP_004525420.1 NZ_CP018386:2962543-2962827 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT69891 NZ_CP018386:2962543-2962827 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAACCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8