Detailed information of TA system TA069888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-MazE
• Location: 75855..76450
• Replicon: plasmid p1
• Accession: NZ_CP018385
• Organism: Burkholderia pseudomallei strain 2008724860

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: BOC44_RS21440
• Protein ID: WP_085513096.1
• Coordinates: 75855..76205 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: -
• Locus tag: BOC44_RS21445
• Protein ID: WP_085513389.1
• Coordinates: 76199..76450 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOC44_RS21400	70863..71198	-	336	WP_085513084.1	hypothetical protein	-
BOC44_RS21410	72053..72328	-	276	WP_157131276.1	hypothetical protein	-
BOC44_RS21420	73183..73410	-	228	WP_085513092.1	hypothetical protein	-
BOC44_RS39240	73424..73591	-	168	WP_157131277.1	hypothetical protein	-
BOC44_RS21425	73605..73904	-	300	WP_085513387.1	hypothetical protein	-
BOC44_RS21430	74362..74760	-	399	WP_085513093.1	hypothetical protein	-
BOC44_RS21435	74786..75172	-	387	WP_157131278.1	hypothetical protein	-
BOC44_RS39245	75191..75454	-	264	WP_157131279.1	hypothetical protein	-
BOC44_RS39250	75686..75844	-	159	WP_157131280.1	hypothetical protein	-
BOC44_RS21440	75855..76205	-	351	WP_085513096.1	type II toxin-antitoxin system ChpB family toxin	Toxin
BOC44_RS21445	76199..76450	-	252	WP_085513389.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BOC44_RS21450	76809..77159	-	351	WP_157131281.1	hypothetical protein	-
BOC44_RS21455	77528..78355	-	828	WP_085513100.1	A24 family peptidase	-
BOC44_RS21460	78390..78695	-	306	WP_085513102.1	hypothetical protein	-
BOC44_RS39255	78685..78996	+	312	WP_157131282.1	hypothetical protein	-
BOC44_RS21465	79086..79358	-	273	WP_085513104.1	hypothetical protein	-
BOC44_RS21470	79595..79969	+	375	WP_085513106.1	hypothetical protein	-
BOC44_RS21475	80108..80380	+	273	WP_085513108.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..297875	297875

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-116)

Antitoxin

• Antitoxin PemI-like (1-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12406.36 Da
• Isoelectric Point: 8.6592

>T69888 WP_085513096.1 NZ_CP018385:c76205-75855 [Burkholderia pseudomallei]
MVKRVKFERGDIVRVSLNPTMGKEQQGDFRPALVLSPAAFNALGVALVAPITQGGEFARYAGFAVPLSGSGTETQGVALV
NMVRTLDLEARGARKVERAPVEVVEDALARLQTILE

Nucleotide

• Length: 351 bp

>T69888 NZ_CP018385:c76205-75855 [Burkholderia pseudomallei]
ATGGTGAAGCGTGTCAAGTTCGAGCGTGGCGATATCGTGCGAGTTAGCCTGAATCCGACGATGGGAAAAGAGCAACAAGG
GGATTTTCGGCCCGCGCTCGTTCTCTCTCCTGCTGCGTTCAACGCGCTCGGCGTGGCGCTAGTCGCGCCGATTACGCAGG
GCGGTGAATTTGCGCGATATGCCGGGTTCGCCGTGCCGCTCTCGGGTTCGGGCACCGAAACGCAAGGCGTTGCGCTCGTG
AACATGGTTCGGACGCTCGATCTTGAGGCGCGTGGCGCACGCAAGGTTGAGCGCGCGCCGGTCGAGGTTGTTGAAGATGC
GCTCGCCCGCCTTCAGACAATTTTGGAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 8828.08 Da
• Isoelectric Point: 4.7063

>AT69888 WP_085513389.1 NZ_CP018385:c76450-76199 [Burkholderia pseudomallei]
MELKIQKWGNSAAVRLPAVLLEQIGASAGSSLTAEVRPDGVLLTPARRKYSLDELVAQCDAKAPEPADLAAWGEVNAVGR
EAW

Nucleotide

• Length: 252 bp

>AT69888 NZ_CP018385:c76450-76199 [Burkholderia pseudomallei]
ATGGAACTCAAGATTCAGAAGTGGGGAAACAGTGCCGCCGTCCGACTTCCCGCCGTGTTACTTGAGCAGATCGGCGCATC
GGCGGGTAGCTCACTGACCGCCGAGGTGCGGCCCGATGGCGTTTTGCTGACGCCCGCTCGTCGCAAGTATTCGCTCGATG
AACTTGTCGCGCAGTGCGACGCGAAGGCCCCGGAACCGGCCGACCTTGCTGCATGGGGTGAAGTCAACGCGGTGGGACGT
GAAGCATGGTGA