Detailed information of TA system TA069882

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 1437890..1438331
• Replicon: chromosome
• Accession: NZ_CP018382
• Organism: Burkholderia pseudomallei strain 2008724758

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: BOC43_RS07670
• Protein ID: WP_009939402.1
• Coordinates: 1437890..1438063 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: BOC43_RS07675
• Protein ID: WP_004525420.1
• Coordinates: 1438047..1438331 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOC43_RS40165	1433768..1434196	-	429	Protein_1216	DDE-type integrase/transposase/recombinase	-
BOC43_RS39430	1434194..1434967	-	774	Protein_1217	prohibitin family protein	-
BOC43_RS40170	1434964..1435113	-	150	WP_004537646.1	bacteriophage protein Gp44	-
BOC43_RS07640	1435372..1435952	+	581	Protein_1219	hypothetical protein	-
BOC43_RS39440	1436183..1436482	-	300	WP_160458000.1	hypothetical protein	-
BOC43_RS07650	1436449..1436670	-	222	WP_004552390.1	hypothetical protein	-
BOC43_RS40175	1436806..1436937	-	132	WP_009923452.1	bacteriophage protein Gp48	-
BOC43_RS07665	1436947..1437222	-	276	WP_085547174.1	hypothetical protein	-
BOC43_RS07670	1437890..1438063	+	174	WP_009939402.1	BrnT family toxin	Toxin
BOC43_RS07675	1438047..1438331	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
BOC43_RS07690	1438978..1439361	+	384	Protein_1226	DDE-type integrase/transposase/recombinase	-
BOC43_RS07695	1439324..1439752	+	429	Protein_1227	IS110 family transposase	-
BOC43_RS07700	1439979..1440095	-	117	Protein_1228	IS3 family transposase	-
BOC43_RS07705	1440342..1441802	+	1461	WP_085547175.1	metallophosphoesterase	-
BOC43_RS07710	1441961..1443223	+	1263	WP_004530738.1	c-type cytochrome	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1427616..1508400	80784

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T69882 WP_009939402.1 NZ_CP018382:1437890-1438063 [Burkholderia pseudomallei]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T69882 NZ_CP018382:1437890-1438063 [Burkholderia pseudomallei]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT69882 WP_004525420.1 NZ_CP018382:1438047-1438331 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT69882 NZ_CP018382:1438047-1438331 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8