Detailed information of TA system TA069881

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1427761..1428400
• Replicon: chromosome
• Accession: NZ_CP018382
• Organism: Burkholderia pseudomallei strain 2008724758

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Q2T3Q6
• Locus tag: BOC43_RS07585
• Protein ID: WP_009893919.1
• Coordinates: 1427761..1427940 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q2T3Q7
• Locus tag: BOC43_RS07590
• Protein ID: WP_011401461.1
• Coordinates: 1427984..1428400 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOC43_RS07555	1423071..1423838	+	768	WP_197690675.1	ATP-binding protein	-
BOC43_RS07560	1423865..1424074	+	210	WP_085547168.1	KTSC domain-containing protein	-
BOC43_RS07565	1424452..1425027	+	576	WP_085547169.1	recombinase family protein	-
BOC43_RS07570	1425098..1426213	-	1116	WP_009893924.1	DUF3396 domain-containing protein	-
BOC43_RS07575	1426223..1426957	-	735	WP_085547170.1	VRR-NUC domain-containing protein	-
BOC43_RS07580	1426954..1427481	-	528	WP_060819804.1	PAAR domain-containing protein	-
BOC43_RS07585	1427761..1427940	+	180	WP_009893919.1	type II toxin-antitoxin system HicA family toxin	Toxin
BOC43_RS07590	1427984..1428400	+	417	WP_011401461.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BOC43_RS07605	1429335..1430906	-	1572	WP_085547171.1	IS66 family transposase	-
BOC43_RS07610	1430936..1431283	-	348	WP_085547172.1	IS66 family insertion sequence element accessory protein TnpB	-
BOC43_RS07615	1431280..1431561	-	282	WP_101562118.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1427616..1508400	80784
	-	flank	IS/Tn	-	-	1421515..1422765	1250

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6618.82 Da
• Isoelectric Point: 11.2336

>T69881 WP_009893919.1 NZ_CP018382:1427761-1427940 [Burkholderia pseudomallei]
MNSSKLIRMLEEDGWRLVRVTGSHHHFKHPKKPGLVTVPHPKKDLPIGTVKSIQKSAGL

Nucleotide

• Length: 180 bp

>T69881 NZ_CP018382:1427761-1427940 [Burkholderia pseudomallei]
ATGAACTCATCGAAGCTGATCCGGATGCTTGAGGAAGATGGCTGGAGGTTGGTTCGAGTGACAGGCAGCCATCATCATTT
CAAACACCCCAAGAAGCCAGGTCTTGTGACGGTTCCCCACCCGAAGAAGGACCTACCGATTGGGACTGTAAAAAGCATCC
AGAAATCCGCCGGCCTGTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.03 Da
• Isoelectric Point: 4.6944

>AT69881 WP_011401461.1 NZ_CP018382:1427984-1428400 [Burkholderia pseudomallei]
MEFPIAVHKDDGSVYGVTVPDIPGVHSWGETIDDAIKNTREAIVGHVETLIELGDDVEFTCSTVEELVAKPEYAGAVWAL
VSVDLSQLDSKPERINVSIPRFVLHKIDAYVASRHETRSGFLARAALEALNEGKVRHA

Nucleotide

• Length: 417 bp

>AT69881 NZ_CP018382:1427984-1428400 [Burkholderia pseudomallei]
ATGGAATTTCCCATCGCAGTGCATAAGGACGATGGGAGCGTCTACGGCGTGACGGTCCCGGATATTCCGGGCGTTCACTC
CTGGGGCGAAACCATCGATGATGCAATCAAAAACACGAGAGAGGCTATCGTCGGCCATGTAGAGACATTGATCGAGCTTG
GGGACGATGTTGAATTCACTTGCTCGACGGTGGAAGAACTGGTTGCAAAGCCCGAGTACGCCGGGGCGGTTTGGGCGCTC
GTCAGCGTTGATCTTTCTCAGCTTGATTCCAAGCCCGAGCGGATCAATGTGAGTATCCCTCGCTTCGTGCTGCACAAGAT
TGACGCCTATGTGGCATCCCGCCACGAGACTAGAAGCGGATTTCTTGCGCGTGCTGCCCTGGAGGCACTTAATGAAGGCA
AAGTTAGGCACGCATAA

Structures

Toxin

• PDB: 4C26
• PDB: 6G26

Antitoxin

• AlphaFold DB: Q2T3Q7