Detailed information of TA system TA069870

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 74998..75801
• Replicon: plasmid pKp_Goe_629-2
• Accession: NZ_CP018366
• Organism: Klebsiella pneumoniae strain Kp_Goe_62629

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: W8E895
• Locus tag: BB790_RS30745
• Protein ID: WP_016479963.1
• Coordinates: 74998..75528 (-)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W8E6Q5
• Locus tag: BB790_RS30750
• Protein ID: WP_022652312.1
• Coordinates: 75532..75801 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB790_RS30705	70213..70503	+	291	WP_004201172.1	co-chaperone GroES	-
BB790_RS30710	70559..72199	+	1641	WP_004201176.1	chaperonin GroEL	-
BB790_RS31925	72388..72564	+	177	Protein_87	IS91 family transposase	-
BB790_RS31930	72825..72944	+	120	Protein_88	IS30 family transposase	-
BB790_RS30730	72954..73703	+	750	Protein_89	IS3 family transposase	-
BB790_RS30735	73850..74866	-	1017	WP_087728544.1	IS5-like element IS5 family transposase	-
BB790_RS30745	74998..75528	-	531	WP_016479963.1	GNAT family N-acetyltransferase	Toxin
BB790_RS30750	75532..75801	-	270	WP_022652312.1	DUF1778 domain-containing protein	Antitoxin
BB790_RS30755	76657..77646	+	990	WP_022652311.1	RepB family plasmid replication initiator protein	-
BB790_RS30760	77942..78681	-	740	Protein_94	site-specific integrase	-
BB790_RS31275	79163..79315	+	153	Protein_95	IS5/IS1182 family transposase	-
BB790_RS30770	79387..80507	+	1121	WP_089046468.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / rmtC / blaNDM-1	htpB	1..94434	94434
	-	inside	IScluster/Tn	-	-	73038..83744	10706

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20350.24 Da
• Isoelectric Point: 6.4907

>T69870 WP_016479963.1 NZ_CP018366:c75528-74998 [Klebsiella pneumoniae]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTREDKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Nucleotide

• Length: 531 bp

>T69870 NZ_CP018366:c75528-74998 [Klebsiella pneumoniae]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGG
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10269.70 Da
• Isoelectric Point: 5.7252

>AT69870 WP_022652312.1 NZ_CP018366:c75801-75532 [Klebsiella pneumoniae]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Nucleotide

• Length: 270 bp

>AT69870 NZ_CP018366:c75801-75532 [Klebsiella pneumoniae]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A6C0L7Q2

Antitoxin

• AlphaFold DB: A0A2Z3XDS5