Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 85920..86563 Replicon plasmid pKp_Goe_629-1
Accession NZ_CP018365
Organism Klebsiella pneumoniae strain Kp_Goe_62629

Toxin (Protein)


Gene name vagD Uniprot ID A0A7Z7T700
Locus tag BB790_RS29190 Protein ID WP_040155119.1
Coordinates 85920..86336 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag BB790_RS29195 Protein ID WP_001261276.1
Coordinates 86333..86563 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB790_RS29165 82700..83397 - 698 Protein_101 IS1 family transposase -
BB790_RS29180 83453..84339 - 887 Protein_102 HlyD family secretion protein -
BB790_RS29185 85001..85879 + 879 WP_009309916.1 restriction endonuclease -
BB790_RS29190 85920..86336 - 417 WP_040155119.1 type II toxin-antitoxin system VapC family toxin Toxin
BB790_RS29195 86333..86563 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BB790_RS29200 86925..87275 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
BB790_RS29205 87325..87687 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
BB790_RS29210 87705..89456 + 1752 WP_004152099.1 arsenite efflux transporter ATPase subunit ArsA -
BB790_RS29215 89504..90793 + 1290 WP_004152098.1 arsenite efflux transporter membrane subunit ArsB -
BB790_RS29220 90806..91231 + 426 WP_004152097.1 glutaredoxin-dependent arsenate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-15 / catA1 / tet(A) / dfrA1 / qacE / sul1 / aac(6')-Ib-cr / blaOXA-1 / aadA2 / mph(A) / aac(3)-IIa - 1..260772 260772
- flank IS/Tn - - 82700..83077 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15147.60 Da        Isoelectric Point: 7.8915

>T69869 WP_040155119.1 NZ_CP018365:c86336-85920 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIHLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLVLEDWVK

Download         Length: 417 bp

>T69869 NZ_CP018365:c86336-85920 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAATCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCATCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT69869 WP_001261276.1 NZ_CP018365:c86563-86333 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT69869 NZ_CP018365:c86563-86333 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z7T700

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References