Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 74979..75715 Replicon plasmid pKp_Goe_629-1
Accession NZ_CP018365
Organism Klebsiella pneumoniae strain Kp_Goe_62629

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag BB790_RS29105 Protein ID WP_003026803.1
Coordinates 74979..75461 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag BB790_RS29110 Protein ID WP_003026799.1
Coordinates 75449..75715 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB790_RS29090 71434..72780 - 1347 WP_020314316.1 ISNCY family transposase -
BB790_RS29095 72981..73376 - 396 WP_004143398.1 helix-turn-helix domain-containing protein -
BB790_RS29100 73425..74771 - 1347 WP_077253535.1 ISNCY family transposase -
BB790_RS29105 74979..75461 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
BB790_RS29110 75449..75715 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
BB790_RS29115 75891..76145 - 255 WP_004152108.1 hypothetical protein -
BB790_RS29120 76221..76478 - 258 WP_004152107.1 hypothetical protein -
BB790_RS29125 76527..76730 - 204 WP_004152106.1 hemolysin expression modulator Hha -
BB790_RS29130 76764..77132 - 369 WP_004152105.1 hypothetical protein -
BB790_RS29135 77176..77670 - 495 WP_004152104.1 DNA-binding protein -
BB790_RS29140 77701..78276 - 576 WP_004152103.1 hypothetical protein -
BB790_RS29145 78264..78533 - 270 WP_004152102.1 hypothetical protein -
BB790_RS29150 79100..79444 + 345 WP_009309911.1 hypothetical protein -
BB790_RS29155 79552..80187 - 636 WP_009309912.1 CPBP family intramembrane metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-15 / catA1 / tet(A) / dfrA1 / qacE / sul1 / aac(6')-Ib-cr / blaOXA-1 / aadA2 / mph(A) / aac(3)-IIa - 1..260772 260772


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T69868 WP_003026803.1 NZ_CP018365:c75461-74979 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T69868 NZ_CP018365:c75461-74979 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT69868 WP_003026799.1 NZ_CP018365:c75715-75449 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT69868 NZ_CP018365:c75715-75449 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References