Detailed information of TA system TA069845

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1214541..1215194
• Replicon: chromosome
• Accession: NZ_CP018363
• Organism: Mycobacterium avium subsp. hominissuis strain H87

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: BS641_RS06155
• Protein ID: WP_003872880.1
• Coordinates: 1214733..1215194 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: Q742J1
• Locus tag: BS641_RS06150
• Protein ID: WP_003872881.1
• Coordinates: 1214541..1214729 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BS641_RS06140	1210523..1212109	+	1587	WP_023900196.1	serine hydrolase	-
BS641_RS06145	1212106..1214478	+	2373	WP_033720313.1	cation-translocating P-type ATPase	-
BS641_RS06150	1214541..1214729	+	189	WP_003872881.1	antitoxin	Antitoxin
BS641_RS06155	1214733..1215194	+	462	WP_003872880.1	SRPBCC family protein	Toxin
BS641_RS06160	1215263..1215697	+	435	WP_003877359.1	hypothetical protein	-
BS641_RS06165	1216694..1218277	+	1584	WP_003872878.1	DUF4185 domain-containing protein	-
BS641_RS06170	1218324..1218710	+	387	WP_033720314.1	hypothetical protein	-
BS641_RS06180	1219497..1219835	+	339	WP_009975166.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (2-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-51)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16626.19 Da
• Isoelectric Point: 9.5654

>T69845 WP_003872880.1 NZ_CP018363:1214733-1215194 [Mycobacterium avium subsp. hominissuis]
VAKLSGSIDVPLPPEVAWQHASDLSRYKEWLTIHRVWRSTLPDQIDKGTVVESIVEVKGMLNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKVQPKADGSVVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFARSDPSTNGHR

Nucleotide

• Length: 462 bp

>T69845 NZ_CP018363:1214733-1215194 [Mycobacterium avium subsp. hominissuis]
GTGGCCAAGCTTTCCGGATCCATCGATGTTCCGCTGCCCCCCGAGGTGGCCTGGCAGCACGCCTCCGACCTGTCCCGGTA
CAAGGAGTGGCTGACCATTCACCGGGTGTGGCGCAGCACCTTGCCCGACCAGATCGACAAGGGCACCGTCGTCGAGTCGA
TCGTCGAGGTCAAGGGCATGCTCAACCGGATCAAATGGACGGTGGTGCGCTACAAGCCACCGGAGGGCATGACGCTCAAC
GGCGACGGCGTCGGCGGGGTCAAGGTCAAGCTGATGGCCAAGGTGCAGCCCAAGGCGGACGGCTCGGTCGTCAGCTTCGA
CGTGCACCTCGGCGGTCCGGCGCTGTTCGGGCCCATCGGCATGATCGTCGCGGCCGCGCTGCGCGGCGACATCGACCAGT
CGCTGGAGAACTTCGTCACGGTGTTCGCCCGATCGGATCCGAGCACCAACGGTCATCGCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6745.42 Da
• Isoelectric Point: 4.4865

>AT69845 WP_003872881.1 NZ_CP018363:1214541-1214729 [Mycobacterium avium subsp. hominissuis]
MGFLDKAKDLLSQNADKVEMAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKATGATETGDQQG

Nucleotide

• Length: 189 bp

>AT69845 NZ_CP018363:1214541-1214729 [Mycobacterium avium subsp. hominissuis]
ATGGGATTCCTGGACAAGGCCAAGGACCTGTTGTCGCAGAACGCCGACAAGGTCGAGATGGCCATCGACAAGGCCGGCGA
GTTCGTCGACGAAAAGACGCAGGGCAAGTACTCCGACACCATCCACCAGGTGCAGGAGCAGGCCAAGAAGGCCACCGGCG
CCACCGAGACCGGCGACCAGCAGGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E2WC18