Detailed information of TA system TA069840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3876589..3877165
• Replicon: chromosome
• Accession: NZ_CP018362
• Organism: Klebsiella oxytoca strain CAV1752

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: AGH21_RS21250
• Protein ID: WP_038424021.1
• Coordinates: 3876589..3876876 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0H3H591
• Locus tag: AGH21_RS21255
• Protein ID: WP_014227757.1
• Coordinates: 3876863..3877165 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AGH21_RS21220	3871931..3872449	-	519	WP_004098240.1	hypothetical protein	-
AGH21_RS21225	3872446..3873246	-	801	WP_014837308.1	winged helix-turn-helix domain-containing protein	-
AGH21_RS21230	3873392..3873976	-	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
AGH21_RS21235	3874124..3874906	-	783	WP_076752474.1	NAD(P)H-dependent oxidoreductase	-
AGH21_RS21240	3874994..3875902	+	909	WP_014837311.1	LysR family transcriptional regulator	-
AGH21_RS21245	3875896..3876339	+	444	WP_014837312.1	FosA family fosfomycin resistance glutathione transferase	-
AGH21_RS21250	3876589..3876876	+	288	WP_038424021.1	BrnT family toxin	Toxin
AGH21_RS21255	3876863..3877165	+	303	WP_014227757.1	BrnA antitoxin family protein	Antitoxin
AGH21_RS21260	3877286..3878629	-	1344	WP_032719935.1	APC family permease	-
AGH21_RS21265	3878626..3879537	-	912	WP_032719936.1	acetamidase/formamidase family protein	-
AGH21_RS21270	3879990..3880325	-	336	WP_014227760.1	endoribonuclease SymE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (38-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11253.72 Da
• Isoelectric Point: 8.5899

>T69840 WP_038424021.1 NZ_CP018362:3876589-3876876 [Klebsiella oxytoca]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTLGLVHGIIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T69840 NZ_CP018362:3876589-3876876 [Klebsiella oxytoca]
ATGCCAATGGAGTTTGAATGGGATGCGAACAAGGCAAGAAGTAATCTAAGAAAACATGGAATCCGGTTTGAGGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTCTCGGCC
TCGTTCATGGGATCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11607.18 Da
• Isoelectric Point: 9.4429

>AT69840 WP_014227757.1 NZ_CP018362:3876863-3877165 [Klebsiella oxytoca]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT69840 NZ_CP018362:3876863-3877165 [Klebsiella oxytoca]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3H591