Detailed information of TA system TA069837

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2943371..2944110
• Replicon: chromosome
• Accession: NZ_CP018362
• Organism: Klebsiella oxytoca strain CAV1752

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: AGH21_RS16720
• Protein ID: WP_076752153.1
• Coordinates: 2943625..2944110 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A5E1AWX8
• Locus tag: AGH21_RS16715
• Protein ID: WP_012540086.1
• Coordinates: 2943371..2943637 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AGH21_RS16685	2938601..2939137	-	537	WP_013087166.1	GNAT family N-acetyltransferase	-
AGH21_RS16690	2939262..2941013	-	1752	WP_187420813.1	arsenical pump-driving ATPase	-
AGH21_RS16695	2941040..2941402	-	363	WP_013087168.1	arsenic metallochaperone ArsD family protein	-
AGH21_RS16700	2941478..2942023	-	546	WP_047365773.1	sigma-70 family RNA polymerase sigma factor	-
AGH21_RS16705	2942032..2942745	-	714	WP_013087170.1	arsenical resistance protein ArsH	-
AGH21_RS16710	2942747..2943070	-	324	WP_013087171.1	metalloregulator ArsR/SmtB family transcription factor	-
AGH21_RS16715	2943371..2943637	+	267	WP_012540086.1	DUF1778 domain-containing protein	Antitoxin
AGH21_RS16720	2943625..2944110	+	486	WP_076752153.1	GNAT family N-acetyltransferase	Toxin
AGH21_RS16725	2944632..2946701	-	2070	WP_025106788.1	glycine--tRNA ligase subunit beta	-
AGH21_RS16730	2946711..2947622	-	912	WP_004126458.1	glycine--tRNA ligase subunit alpha	-
AGH21_RS16735	2947750..2948055	-	306	WP_014227200.1	YsaB family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2931529..2944110	12581

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17838.68 Da
• Isoelectric Point: 10.0748

>T69837 WP_076752153.1 NZ_CP018362:2943625-2944110 [Klebsiella oxytoca]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSRTQQRTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T69837 NZ_CP018362:2943625-2944110 [Klebsiella oxytoca]
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTTATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACGAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10076.47 Da
• Isoelectric Point: 4.3721

>AT69837 WP_012540086.1 NZ_CP018362:2943371-2943637 [Klebsiella oxytoca]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT69837 NZ_CP018362:2943371-2943637 [Klebsiella oxytoca]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCAAAGGAATCACAGCGGGCGCTCATTGATGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAACGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A5E1AWX8