69835

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1118555..1119145 Replicon chromosome
Accession NZ_CP018362
Organism Klebsiella oxytoca strain CAV1752

Toxin (Protein)


Gene name pemK Uniprot ID A0A0H3HDW1
Locus tag AGH21_RS07855 Protein ID WP_014229979.1
Coordinates 1118555..1118887 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag AGH21_RS07860 Protein ID WP_004852307.1
Coordinates 1118888..1119145 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGH21_RS07830 1113968..1114225 + 258 WP_014229908.1 histidine kinase -
AGH21_RS07835 1114358..1115812 + 1455 WP_014229909.1 AMP nucleosidase -
AGH21_RS07845 1116775..1118232 - 1458 WP_014229916.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
AGH21_RS07855 1118555..1118887 - 333 WP_014229979.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AGH21_RS07860 1118888..1119145 - 258 WP_004852307.1 antitoxin Antitoxin
AGH21_RS31850 1119856..1119936 + 81 Protein_1067 molybdopterin-binding protein -
AGH21_RS07880 1120127..1121041 - 915 WP_014229981.1 LysR family transcriptional regulator -
AGH21_RS07885 1121142..1122140 + 999 WP_014229982.1 aldo/keto reductase -
AGH21_RS07890 1122178..1123104 - 927 WP_014229983.1 LysR family transcriptional regulator -
AGH21_RS07895 1123281..1123889 + 609 WP_014229984.1 glutathione S-transferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11740.57 Da        Isoelectric Point: 10.1863

>T69835 WP_014229979.1 NZ_CP018362:c1118887-1118555 [Klebsiella oxytoca]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMAARNGKRLESIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T69835 NZ_CP018362:c1118887-1118555 [Klebsiella oxytoca]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGTTACCCGTTGTTGTTCCCATCACCAGCGGTGGAAATTTTGCTCGCA
CGGCAGGTTTCACCGTTTCACTGGAAGGGGCAGGGACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGCTGCCCGGAACGGCAAGCGGCTGGAAAGCATACCAGACGCTGTGGTAAATGAAGTGCTTGCCAGGCTGGATGC
AATTTTAAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT69835 WP_004852307.1 NZ_CP018362:c1119145-1118888 [Klebsiella oxytoca]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT69835 NZ_CP018362:c1119145-1118888 [Klebsiella oxytoca]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGTTGGCGGTTCCTCCCGCCGTGCTCAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTCGGCATGACCATTAATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCTCATTATTCTC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCTCCTGCGGTG
GGCAAGGAGATCCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3HDW1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References