Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 129825..130468 Replicon plasmid pCAV1752-278
Accession NZ_CP018361
Organism Klebsiella oxytoca strain CAV1752

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag AGH21_RS01500 Protein ID WP_001044770.1
Coordinates 130052..130468 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag AGH21_RS01495 Protein ID WP_001261282.1
Coordinates 129825..130055 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGH21_RS32230 125987..126100 + 114 WP_012540012.1 hypothetical protein -
AGH21_RS01460 126229..126486 + 258 WP_000764642.1 hypothetical protein -
AGH21_RS01465 126544..127323 - 780 WP_029497485.1 site-specific integrase -
AGH21_RS01470 127521..128525 - 1005 WP_029497484.1 hypothetical protein -
AGH21_RS01475 128712..129017 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB -
AGH21_RS01480 129019..129237 - 219 WP_004197642.1 type II toxin-antitoxin system antitoxin CcdA -
AGH21_RS01490 129407..129868 - 462 WP_160866775.1 hypothetical protein -
AGH21_RS01495 129825..130055 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AGH21_RS01500 130052..130468 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
AGH21_RS01505 130542..132104 + 1563 WP_004206609.1 AAA family ATPase -
AGH21_RS01510 132089..133111 + 1023 WP_000361404.1 helicase UvrD -
AGH21_RS01520 133356..134053 + 698 Protein_138 IS1 family transposase -
AGH21_RS01525 134070..134879 + 810 Protein_139 GGDEF domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..277616 277616


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T69824 WP_001044770.1 NZ_CP018361:130052-130468 [Klebsiella oxytoca]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T69824 NZ_CP018361:130052-130468 [Klebsiella oxytoca]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT69824 WP_001261282.1 NZ_CP018361:129825-130055 [Klebsiella oxytoca]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT69824 NZ_CP018361:129825-130055 [Klebsiella oxytoca]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References