Detailed information of TA system TA069823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 128712..129237
• Replicon: plasmid pCAV1752-278
• Accession: NZ_CP018361
• Organism: Klebsiella oxytoca strain CAV1752

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: AGH21_RS01475
• Protein ID: WP_013023785.1
• Coordinates: 128712..129017 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: AGH21_RS01480
• Protein ID: WP_004197642.1
• Coordinates: 129019..129237 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AGH21_RS01455	124598..125353	-	756	WP_012539983.1	replication initiation protein RepE	-
AGH21_RS32230	125987..126100	+	114	WP_012540012.1	hypothetical protein	-
AGH21_RS01460	126229..126486	+	258	WP_000764642.1	hypothetical protein	-
AGH21_RS01465	126544..127323	-	780	WP_029497485.1	site-specific integrase	-
AGH21_RS01470	127521..128525	-	1005	WP_029497484.1	hypothetical protein	-
AGH21_RS01475	128712..129017	-	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
AGH21_RS01480	129019..129237	-	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
AGH21_RS01490	129407..129868	-	462	WP_160866775.1	hypothetical protein	-
AGH21_RS01495	129825..130055	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
AGH21_RS01500	130052..130468	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	-
AGH21_RS01505	130542..132104	+	1563	WP_004206609.1	AAA family ATPase	-
AGH21_RS01510	132089..133111	+	1023	WP_000361404.1	helicase UvrD	-
AGH21_RS01520	133356..134053	+	698	Protein_138	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..277616	277616

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T69823 WP_013023785.1 NZ_CP018361:c129017-128712 [Klebsiella oxytoca]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T69823 NZ_CP018361:c129017-128712 [Klebsiella oxytoca]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT69823 WP_004197642.1 NZ_CP018361:c129237-129019 [Klebsiella oxytoca]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT69823 NZ_CP018361:c129237-129019 [Klebsiella oxytoca]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6