Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 99658..100184 Replicon plasmid pCAV1752-278
Accession NZ_CP018361
Organism Klebsiella oxytoca strain CAV1752

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag AGH21_RS01290 Protein ID WP_000323025.1
Coordinates 99658..99945 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag AGH21_RS01295 Protein ID WP_000534858.1
Coordinates 99945..100184 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGH21_RS01250 95152..96200 + 1049 Protein_96 IS3 family transposase -
AGH21_RS01275 97597..98454 - 858 WP_020316930.1 incFII family plasmid replication initiator RepA -
AGH21_RS31655 98447..98494 - 48 WP_073984493.1 hypothetical protein -
AGH21_RS01285 98557..99591 + 1035 Protein_99 IS481 family transposase -
AGH21_RS01290 99658..99945 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
AGH21_RS01295 99945..100184 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
AGH21_RS01300 100313..101473 - 1161 WP_076752093.1 IS30 family transposase -
AGH21_RS01305 101670..102593 - 924 WP_020277922.1 cation diffusion facilitator family transporter -
AGH21_RS01310 102793..103365 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
AGH21_RS01320 103841..105079 - 1239 WP_029497477.1 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..277616 277616
- inside IScluster/Tn - - 90570..101473 10903


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T69822 WP_000323025.1 NZ_CP018361:c99945-99658 [Klebsiella oxytoca]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T69822 NZ_CP018361:c99945-99658 [Klebsiella oxytoca]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT69822 WP_000534858.1 NZ_CP018361:c100184-99945 [Klebsiella oxytoca]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT69822 NZ_CP018361:c100184-99945 [Klebsiella oxytoca]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References