Detailed information of TA system TA069821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/FR47-DUF1778
• Location: 22674..23469
• Replicon: plasmid pCAV1752-111
• Accession: NZ_CP018360
• Organism: Klebsiella oxytoca strain CAV1752

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: AGH21_RS00175
• Protein ID: WP_076751958.1
• Coordinates: 22674..23192 (-)
• Length: 173 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: AGH21_RS00180
• Protein ID: WP_064383558.1
• Coordinates: 23197..23469 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AGH21_RS00150	19219..20763	+	1545	WP_076751949.1	tail fiber domain-containing protein	-
AGH21_RS00155	20846..21160	+	315	WP_009484919.1	hypothetical protein	-
AGH21_RS00160	21171..21863	+	693	WP_076751951.1	hypothetical protein	-
AGH21_RS00165	21860..22111	+	252	WP_076751953.1	hypothetical protein	-
AGH21_RS00170	22334..22543	-	210	WP_076751956.1	hypothetical protein	-
AGH21_RS00175	22674..23192	-	519	WP_076751958.1	GNAT family N-acetyltransferase	Toxin
AGH21_RS00180	23197..23469	-	273	WP_064383558.1	DUF1778 domain-containing protein	Antitoxin
AGH21_RS00185	23778..24443	+	666	WP_047719673.1	AAA family ATPase	-
AGH21_RS00190	24448..24822	+	375	WP_047719674.1	hypothetical protein	-
AGH21_RS00195	25151..25897	+	747	WP_076751960.1	hypothetical protein	-
AGH21_RS00200	25936..27297	+	1362	WP_047719676.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..111017	111017

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (50-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-88)

Sequences

Toxin

Protein

• Length: 173 a.a.
• Molecular weight: 19354.16 Da
• Isoelectric Point: 7.1047

>T69821 WP_076751958.1 NZ_CP018360:c23192-22674 [Klebsiella oxytoca]
VNTYDIEPIQKDFPYDISSFDCGNEALNLFLSEHLQRQDEKQILRGYVYLEYGSGLPKVLGFFTLAGGSFERSRFPSKSQ
QKKIPYTNTPCIILGRLAVDKTIQRQGIGEMLVVKAAKIVLQSANAIGLHGMFVDAKNDDACKFYENLGFTRLIGDNENL
FFYPTSKIAQLP

Nucleotide

• Length: 519 bp

>T69821 NZ_CP018360:c23192-22674 [Klebsiella oxytoca]
GTGAACACGTATGATATTGAACCAATACAGAAAGATTTTCCATACGACATTAGCTCCTTCGACTGCGGTAACGAGGCGCT
AAATTTATTCCTGTCAGAACATCTTCAAAGACAGGACGAAAAGCAGATTCTGCGTGGATACGTATATCTTGAGTATGGAT
CTGGCTTACCAAAAGTGCTTGGCTTCTTTACTTTGGCCGGTGGGTCGTTTGAGAGAAGCCGATTTCCGTCCAAAAGCCAG
CAGAAGAAAATTCCTTACACTAATACCCCATGCATAATCTTAGGGCGACTGGCTGTTGACAAAACAATCCAGCGACAAGG
TATCGGGGAAATGCTGGTAGTCAAGGCTGCTAAAATAGTTCTCCAATCCGCTAACGCAATAGGTCTTCACGGTATGTTTG
TTGATGCGAAAAATGATGATGCCTGTAAATTTTATGAGAATTTAGGGTTTACTAGGTTGATTGGTGATAATGAAAACCTA
TTCTTTTACCCTACATCCAAAATTGCACAGCTCCCATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10317.85 Da
• Isoelectric Point: 6.9840

>AT69821 WP_064383558.1 NZ_CP018360:c23469-23197 [Klebsiella oxytoca]
MPTLKKERIDIRLTDEAKSVIEEAAILSNLSVSQFVISSAAERATEVIEQHRRIVLCEDSWKLVMDAINNPDQPNDRMKR
AAERLKNLRT

Nucleotide

• Length: 273 bp

>AT69821 NZ_CP018360:c23469-23197 [Klebsiella oxytoca]
ATGCCCACTTTGAAAAAAGAACGTATCGATATAAGACTGACCGATGAAGCTAAGTCAGTCATTGAGGAAGCGGCGATCTT
GTCCAATCTAAGCGTGAGCCAGTTTGTGATAAGTAGCGCAGCAGAAAGAGCCACAGAGGTGATTGAGCAGCATCGCAGAA
TCGTTTTGTGCGAAGATTCCTGGAAATTGGTGATGGATGCCATCAACAACCCAGATCAACCGAATGACAGAATGAAACGA
GCTGCTGAGCGCCTTAAAAATTTAAGGACCTGA