69817

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4613300..4613925 Replicon chromosome
Accession NZ_CP018356
Organism Klebsiella pneumoniae strain CAV1453

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag AGG69_RS25895 Protein ID WP_002882817.1
Coordinates 4613542..4613925 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag AGG69_RS25890 Protein ID WP_004150355.1
Coordinates 4613300..4613542 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGG69_RS25865 4609291..4609890 + 600 WP_004151865.1 glucose-1-phosphatase -
AGG69_RS25870 4609884..4610744 + 861 WP_002882807.1 virulence factor BrkB family protein -
AGG69_RS25875 4610741..4611178 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
AGG69_RS25880 4611223..4612164 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
AGG69_RS25885 4612178..4613095 - 918 WP_002882812.1 alpha/beta hydrolase -
AGG69_RS25890 4613300..4613542 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
AGG69_RS25895 4613542..4613925 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
AGG69_RS25900 4614099..4615028 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
AGG69_RS25905 4615025..4615660 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
AGG69_RS25910 4615657..4616559 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T69817 WP_002882817.1 NZ_CP018356:4613542-4613925 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T69817 NZ_CP018356:4613542-4613925 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT69817 WP_004150355.1 NZ_CP018356:4613300-4613542 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT69817 NZ_CP018356:4613300-4613542 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References