Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 188817..189553 Replicon plasmid pCAV1453-208
Accession NZ_CP018355
Organism Klebsiella pneumoniae strain CAV1453

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AGG69_RS01590 Protein ID WP_003026803.1
Coordinates 188817..189299 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AGG69_RS01595 Protein ID WP_003026799.1
Coordinates 189287..189553 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGG69_RS01570 184454..185416 + 963 WP_004152113.1 M48 family metalloprotease -
AGG69_RS30205 185504..186758 + 1255 Protein_188 IS3 family transposase -
AGG69_RS01585 187259..188605 - 1347 WP_020314316.1 ISNCY family transposase -
AGG69_RS01590 188817..189299 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AGG69_RS01595 189287..189553 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AGG69_RS01600 189729..189983 - 255 WP_004152108.1 hypothetical protein -
AGG69_RS01605 190059..190316 - 258 WP_004152107.1 hypothetical protein -
AGG69_RS01610 190365..190568 - 204 WP_004152106.1 hemolysin expression modulator Hha -
AGG69_RS01615 190602..190970 - 369 WP_004152105.1 hypothetical protein -
AGG69_RS01620 191014..191508 - 495 WP_004152104.1 DNA-binding protein -
AGG69_RS01625 191539..192114 - 576 WP_004152103.1 hypothetical protein -
AGG69_RS01630 192102..192371 - 270 WP_004152102.1 hypothetical protein -
AGG69_RS01635 192729..193079 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
AGG69_RS01640 193129..193491 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA1 / dfrA12 / aadA2 / qacE / sul1 / mph(A) / aph(3')-Ia - 1..207543 207543


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T69802 WP_003026803.1 NZ_CP018355:c189299-188817 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T69802 NZ_CP018355:c189299-188817 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT69802 WP_003026799.1 NZ_CP018355:c189553-189287 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT69802 NZ_CP018355:c189553-189287 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References