Detailed information of TA system TA069802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 188817..189553
• Replicon: plasmid pCAV1453-208
• Accession: NZ_CP018355
• Organism: Klebsiella pneumoniae strain CAV1453

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: AGG69_RS01590
• Protein ID: WP_003026803.1
• Coordinates: 188817..189299 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: AGG69_RS01595
• Protein ID: WP_003026799.1
• Coordinates: 189287..189553 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AGG69_RS01570	184454..185416	+	963	WP_004152113.1	M48 family metalloprotease	-
AGG69_RS30205	185504..186758	+	1255	Protein_188	IS3 family transposase	-
AGG69_RS01585	187259..188605	-	1347	WP_020314316.1	ISNCY family transposase	-
AGG69_RS01590	188817..189299	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
AGG69_RS01595	189287..189553	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
AGG69_RS01600	189729..189983	-	255	WP_004152108.1	hypothetical protein	-
AGG69_RS01605	190059..190316	-	258	WP_004152107.1	hypothetical protein	-
AGG69_RS01610	190365..190568	-	204	WP_004152106.1	hemolysin expression modulator Hha	-
AGG69_RS01615	190602..190970	-	369	WP_004152105.1	hypothetical protein	-
AGG69_RS01620	191014..191508	-	495	WP_004152104.1	DNA-binding protein	-
AGG69_RS01625	191539..192114	-	576	WP_004152103.1	hypothetical protein	-
AGG69_RS01630	192102..192371	-	270	WP_004152102.1	hypothetical protein	-
AGG69_RS01635	192729..193079	+	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
AGG69_RS01640	193129..193491	+	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA1 / dfrA12 / aadA2 / qacE / sul1 / mph(A) / aph(3')-Ia	-	1..207543	207543

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T69802 WP_003026803.1 NZ_CP018355:c189299-188817 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T69802 NZ_CP018355:c189299-188817 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT69802 WP_003026799.1 NZ_CP018355:c189553-189287 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT69802 NZ_CP018355:c189553-189287 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66