Detailed information of TA system TA069800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5089553..5090076
• Replicon: chromosome
• Accession: NZ_CP018352
• Organism: Klebsiella pneumoniae strain CAV1417

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V5RNQ6
• Locus tag: AGG42_RS28065
• Protein ID: WP_015632464.1
• Coordinates: 5089771..5090076 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V5RQR8
• Locus tag: AGG42_RS28060
• Protein ID: WP_015632465.1
• Coordinates: 5089553..5089771 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AGG42_RS28040	5085783..5086658	-	876	WP_004098982.1	RepB family plasmid replication initiator protein	-
AGG42_RS28045	5087070..5088341	-	1272	WP_016162068.1	Y-family DNA polymerase	-
AGG42_RS28050	5088341..5088772	-	432	WP_015632467.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
AGG42_RS28055	5088936..5089421	-	486	Protein_4957	IS5 family transposase	-
AGG42_RS28060	5089553..5089771	+	219	WP_015632465.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
AGG42_RS28065	5089771..5090076	+	306	WP_015632464.1	type II toxin-antitoxin system toxin CcdB	Toxin
AGG42_RS28070	5090221..5090565	+	345	WP_015632463.1	hypothetical protein	-
AGG42_RS28075	5090607..5091173	+	567	WP_015632462.1	hypothetical protein	-
AGG42_RS28080	5091377..5091634	-	258	WP_016162071.1	helix-turn-helix domain-containing protein	-
AGG42_RS28085	5092064..5092291	+	228	WP_016162072.1	hypothetical protein	-
AGG42_RS28090	5092305..5092427	+	123	Protein_4964	transcriptional regulator	-
AGG42_RS28095	5092633..5093076	-	444	WP_015632459.1	type II toxin-antitoxin system HicB family antitoxin	-
AGG42_RS28100	5093102..5093284	-	183	WP_015632458.1	type II toxin-antitoxin system HicA family toxin	-
AGG42_RS28105	5093645..5094625	+	981	WP_015632457.1	partitioning protein	-
AGG42_RS28110	5094618..5095046	+	429	WP_015632456.1	plasmid partitioning/stability family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	qacE / sul1 / blaCTX-M-15	-	5054357..5108847	54490

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11361.90 Da
• Isoelectric Point: 5.5252

>T69800 WP_015632464.1 NZ_CP018352:5089771-5090076 [Klebsiella pneumoniae]
MQFTVYEYTRESHYRLFVDVQSDIVETPGRRIVIPLLESHHLSEKVNQQLFPVVSVNGKEYRAVTSELSGVSVSVIGDAV
ADLSHEANAIKNALNLMFWGI

Nucleotide

• Length: 306 bp

>T69800 NZ_CP018352:5089771-5090076 [Klebsiella pneumoniae]
ATGCAGTTCACCGTCTACGAATACACACGCGAGAGCCATTATCGTCTTTTTGTGGATGTGCAGAGCGACATTGTCGAGAC
GCCTGGGCGACGCATAGTGATCCCGTTGCTGGAATCGCACCACTTATCAGAAAAGGTTAATCAGCAGCTTTTCCCGGTGG
TTAGCGTCAATGGGAAAGAATACCGTGCCGTGACTTCCGAATTATCCGGAGTCTCTGTCAGTGTCATCGGCGACGCTGTG
GCCGATCTCAGCCATGAGGCAAATGCCATTAAAAATGCCCTGAACCTGATGTTCTGGGGCATTTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8194.13 Da
• Isoelectric Point: 6.2224

>AT69800 WP_015632465.1 NZ_CP018352:5089553-5089771 [Klebsiella pneumoniae]
MKHRASVTVDKEHYLELLAAGVNISGLVNEAIGREARRIQAERWKAENREGMEEVANFIAQHGSFADENRSW

Nucleotide

• Length: 219 bp

>AT69800 NZ_CP018352:5089553-5089771 [Klebsiella pneumoniae]
ATGAAACACAGAGCCAGCGTCACAGTAGACAAGGAACATTATCTGGAATTACTCGCGGCAGGCGTGAATATTTCGGGACT
GGTGAATGAGGCTATCGGACGGGAAGCCCGGCGCATCCAGGCTGAACGCTGGAAAGCAGAGAACCGGGAAGGGATGGAAG
AGGTAGCGAATTTCATCGCGCAGCACGGCTCTTTTGCCGACGAAAACCGGAGCTGGTGA

Structures

Toxin

• AlphaFold DB: A0A6C0ND19

Antitoxin

• AlphaFold DB: A0A6C0L1T7