Detailed information of TA system TA069790

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1563873..1564396
• Replicon: chromosome
• Accession: NZ_CP018352
• Organism: Klebsiella pneumoniae strain CAV1417

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V5RNQ6
• Locus tag: AGG42_RS10030
• Protein ID: WP_015632464.1
• Coordinates: 1564091..1564396 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V5RQR8
• Locus tag: AGG42_RS10025
• Protein ID: WP_015632465.1
• Coordinates: 1563873..1564091 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AGG42_RS10005	1560103..1560978	-	876	WP_004098982.1	RepB family plasmid replication initiator protein	-
AGG42_RS10010	1561390..1562661	-	1272	WP_016162068.1	Y-family DNA polymerase	-
AGG42_RS10015	1562661..1563092	-	432	WP_015632467.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
AGG42_RS10020	1563256..1563741	-	486	Protein_1577	IS5 family transposase	-
AGG42_RS10025	1563873..1564091	+	219	WP_015632465.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
AGG42_RS10030	1564091..1564396	+	306	WP_015632464.1	type II toxin-antitoxin system toxin CcdB	Toxin
AGG42_RS10035	1564541..1564885	+	345	WP_015632463.1	hypothetical protein	-
AGG42_RS10040	1564927..1565493	+	567	WP_015632462.1	hypothetical protein	-
AGG42_RS10045	1565697..1565954	-	258	WP_016162071.1	helix-turn-helix domain-containing protein	-
AGG42_RS10050	1566384..1566611	+	228	WP_016162072.1	hypothetical protein	-
AGG42_RS10055	1566625..1566747	+	123	Protein_1584	transcriptional regulator	-
AGG42_RS10060	1566953..1567396	-	444	WP_015632459.1	type II toxin-antitoxin system HicB family antitoxin	-
AGG42_RS10065	1567422..1567604	-	183	WP_015632458.1	type II toxin-antitoxin system HicA family toxin	-
AGG42_RS10070	1567965..1568945	+	981	WP_015632457.1	partitioning protein	-
AGG42_RS10075	1568938..1569366	+	429	WP_015632456.1	plasmid partitioning/stability family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	qacE / sul1 / blaCTX-M-15	ompA / nueA / msbA	1502103..1665764	163661

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11361.90 Da
• Isoelectric Point: 5.5252

>T69790 WP_015632464.1 NZ_CP018352:1564091-1564396 [Klebsiella pneumoniae]
MQFTVYEYTRESHYRLFVDVQSDIVETPGRRIVIPLLESHHLSEKVNQQLFPVVSVNGKEYRAVTSELSGVSVSVIGDAV
ADLSHEANAIKNALNLMFWGI

Nucleotide

• Length: 306 bp

>T69790 NZ_CP018352:1564091-1564396 [Klebsiella pneumoniae]
ATGCAGTTCACCGTCTACGAATACACACGCGAGAGCCATTATCGTCTTTTTGTGGATGTGCAGAGCGACATTGTCGAGAC
GCCTGGGCGACGCATAGTGATCCCGTTGCTGGAATCGCACCACTTATCAGAAAAGGTTAATCAGCAGCTTTTCCCGGTGG
TTAGCGTCAATGGGAAAGAATACCGTGCCGTGACTTCCGAATTATCCGGAGTCTCTGTCAGTGTCATCGGCGACGCTGTG
GCCGATCTCAGCCATGAGGCAAATGCCATTAAAAATGCCCTGAACCTGATGTTCTGGGGCATTTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8194.13 Da
• Isoelectric Point: 6.2224

>AT69790 WP_015632465.1 NZ_CP018352:1563873-1564091 [Klebsiella pneumoniae]
MKHRASVTVDKEHYLELLAAGVNISGLVNEAIGREARRIQAERWKAENREGMEEVANFIAQHGSFADENRSW

Nucleotide

• Length: 219 bp

>AT69790 NZ_CP018352:1563873-1564091 [Klebsiella pneumoniae]
ATGAAACACAGAGCCAGCGTCACAGTAGACAAGGAACATTATCTGGAATTACTCGCGGCAGGCGTGAATATTTCGGGACT
GGTGAATGAGGCTATCGGACGGGAAGCCCGGCGCATCCAGGCTGAACGCTGGAAAGCAGAGAACCGGGAAGGGATGGAAG
AGGTAGCGAATTTCATCGCGCAGCACGGCTCTTTTGCCGACGAAAACCGGAGCTGGTGA

Structures

Toxin

• AlphaFold DB: A0A6C0ND19

Antitoxin

• AlphaFold DB: A0A6C0L1T7