Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 79411..80147 Replicon plasmid pCAV1417-185
Accession NZ_CP018351
Organism Klebsiella pneumoniae strain CAV1417

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AGG42_RS01260 Protein ID WP_003026803.1
Coordinates 79665..80147 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AGG42_RS01255 Protein ID WP_003026799.1
Coordinates 79411..79677 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGG42_RS01210 75473..75835 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
AGG42_RS01215 75885..76235 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
AGG42_RS01220 76593..76862 + 270 WP_004152102.1 hypothetical protein -
AGG42_RS01225 76850..77425 + 576 WP_004152103.1 hypothetical protein -
AGG42_RS01230 77456..77950 + 495 WP_004152104.1 DNA-binding protein -
AGG42_RS01235 77994..78362 + 369 WP_004152105.1 hypothetical protein -
AGG42_RS01240 78396..78599 + 204 WP_004152106.1 hemolysin expression modulator Hha -
AGG42_RS01245 78648..78905 + 258 WP_004152107.1 hypothetical protein -
AGG42_RS01250 78981..79235 + 255 WP_004152108.1 hypothetical protein -
AGG42_RS01255 79411..79677 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AGG42_RS01260 79665..80147 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AGG42_RS01265 80359..81705 + 1347 WP_020314316.1 ISNCY family transposase -
AGG42_RS01270 82206..83460 - 1255 Protein_96 IS3 family transposase -
AGG42_RS01275 83548..84510 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..184940 184940


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T69780 WP_003026803.1 NZ_CP018351:79665-80147 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T69780 NZ_CP018351:79665-80147 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT69780 WP_003026799.1 NZ_CP018351:79411-79677 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT69780 NZ_CP018351:79411-79677 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References