Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 47503..48028 Replicon plasmid pKp_Goe_414-3
Accession NZ_CP018340
Organism Klebsiella pneumoniae isolate Kp_Goe_154414

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag BB788_RS29420 Protein ID WP_004197633.1
Coordinates 47723..48028 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag BB788_RS29415 Protein ID WP_001568025.1
Coordinates 47503..47721 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB788_RS29355 42922..43167 + 246 WP_032454911.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
BB788_RS29360 43167..43499 + 333 WP_059065587.1 endoribonuclease MazF -
BB788_RS29365 43759..44082 + 324 WP_073558199.1 hypothetical protein -
BB788_RS29370 44265..44519 - 255 WP_073558200.1 hypothetical protein -
BB788_RS29375 44595..44852 - 258 WP_073558201.1 hypothetical protein -
BB788_RS29380 44901..45104 - 204 WP_004152106.1 hemolysin expression modulator Hha -
BB788_RS29385 45138..45506 - 369 WP_073558202.1 hypothetical protein -
BB788_RS29390 45550..46044 - 495 WP_073558203.1 DNA-binding protein -
BB788_RS29395 46075..46650 - 576 WP_073558204.1 hypothetical protein -
BB788_RS29400 46638..46907 - 270 WP_073558205.1 hypothetical protein -
BB788_RS29415 47503..47721 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
BB788_RS29420 47723..48028 + 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
BB788_RS29425 48197..48598 + 402 WP_073558206.1 hypothetical protein -
BB788_RS29430 48625..48948 + 324 WP_004197641.1 hypothetical protein -
BB788_RS29435 48945..49961 + 1017 WP_046664221.1 hypothetical protein -
BB788_RS29445 50159..50944 + 786 WP_046664219.1 site-specific integrase -
BB788_RS29450 51393..52148 + 756 WP_001568031.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..81939 81939


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T69772 WP_004197633.1 NZ_CP018340:47723-48028 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T69772 NZ_CP018340:47723-48028 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGTTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT69772 WP_001568025.1 NZ_CP018340:47503-47721 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT69772 NZ_CP018340:47503-47721 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References